S100A9 Antibody (S100A9/1011) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11453
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Kappa Clone # S100A9/1011
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for S100A9 Antibody (S100A9/1011) - Azide and BSA Free
Immunogen
Recombinant human S100A9 protein (Uniprot: P06702)
Localization
Cytoplasmic, Extracellular (secreted) and Cell Surface
Specificity
Recognizes a 14kDa protein, identified as S100A9 (also known as Calgranulin B or MRP-14); expressed by granulocytes, monocytes and by tissue macrophages. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Altered expression of this protein is associated with the disease cystic fibrosis. This monoclonal antibody reacts with neutrophils, monocytes and macrophages, and has been shown as an important marker for identifying macrophages in tissue sections. Among cells that are now recognized as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).
Marker
Macrophage Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgM Kappa
Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate. Prepared in 10mM PBS WITHOUT BSA. Also available at 200 ug/ml WITH BSA & azide (NBP2-44910).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Applications for S100A9 Antibody (S100A9/1011) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
0.5 - 1 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed. Not suitable for frozen tissues): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein L or PEG purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: S100A9
Long Name
S100 Calcium Binding Protein A9
Alternate Names
Calgranulin B, MRP-14
Gene Symbol
S100A9
Additional S100A9 Products
Product Documents for S100A9 Antibody (S100A9/1011) - Azide and BSA Free
Product Specific Notices for S100A9 Antibody (S100A9/1011) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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