S100A9 Antibody (S100A9/1075)

Validated:

Unconjugated

Monoclonal Mouse IgG₁ kappa Clone # S100A9/1075

0.2 mg/ml

Recombinant human S100A9 protein (Uniprot: P06702)

Cytoplasmic, Extracellular (secreted) and Cell Surface

This monoclonal antibody stains the cytoplasm of macrophages and histiocytes in hematopoietic organs, Kupffers cells of the liver and Langerhans cells of the skin. It also stains the mantle zone B-lymphocytes of the lymph node and spleen, spermatogonia, and chief cells of the stomach. S100A9 is expressed by macrophages in acutely inflamed tissues and in chronic inflammation. It is detected in peripheral blood leukocytes, in neutrophils and granulocytes. It is present at sites of vascular inflammation. S100A9 is also expressed in epithelial cells constitutively or induced during dermatoses. S100A9 is a Calcium-binding protein. It has antimicrobial activity towards bacteria and fungi. It is important for resistance to invasion by pathogenic bacteria. It up-regulates transcription of genes that are under the control of NF-kappa-B. S100A9 plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS). It promotes tubulin polymerization when unphosphorylated. It also promotes phagocyte migration and infiltration of granulocytes at sites of wounding. It plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1.

Macrophage Marker

Monoclonal

Mouse

IgG₁ kappa

14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% azide. Also available WITHOUT azide at 1.0 mg/ml. (NBP3-11559)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Protein A or G purified

10 mM PBS

0.05% Sodium Azide

0.2 mg/ml

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C.