S100A9 Antibody (S100A9/1075)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44913
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # S100A9/1075
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant human S100A9 protein (Uniprot: P06702)
Localization
Cytoplasmic, Extracellular (secreted) and Cell Surface
Specificity
This monoclonal antibody stains the cytoplasm of macrophages and histiocytes in hematopoietic organs, Kupffers cells of the liver and Langerhans cells of the skin. It also stains the mantle zone B-lymphocytes of the lymph node and spleen, spermatogonia, and chief cells of the stomach. S100A9 is expressed by macrophages in acutely inflamed tissues and in chronic inflammation. It is detected in peripheral blood leukocytes, in neutrophils and granulocytes. It is present at sites of vascular inflammation. S100A9 is also expressed in epithelial cells constitutively or induced during dermatoses. S100A9 is a Calcium-binding protein. It has antimicrobial activity towards bacteria and fungi. It is important for resistance to invasion by pathogenic bacteria. It up-regulates transcription of genes that are under the control of NF-kappa-B. S100A9 plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS). It promotes tubulin polymerization when unphosphorylated. It also promotes phagocyte migration and infiltration of granulocytes at sites of wounding. It plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1.
Marker
Macrophage Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% azide. Also available WITHOUT azide at 1.0 mg/ml. (NBP3-11559)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for S100A9 Antibody (S100A9/1075)
Immunohistochemistry-Paraffin: S100A9 Antibody (S100A9/1075) [NBP2-44913]
Immunohistochemistry-Paraffin: S100A9 Antibody (S100A9/1075) [NBP2-44913] - Formalin-fixed, paraffin-embedded human skin carcinoma stained with S100A9 Antibody (S100A9/1075).Immunohistochemistry-Paraffin: S100A9 Antibody (S100A9/1075) [NBP2-44913]
Immunohistochemistry-Paraffin: S100A9 Antibody (S100A9/1075) [NBP2-44913] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with S100A9 Antibody (S100A9/1075).Applications for S100A9 Antibody (S100A9/1075)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. For staining of formalin-fixed, paraffin-embedded tissues, digest with trypsin at 1mg/ml PBS, 15 min at RT.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: S100A9
Long Name
S100 Calcium Binding Protein A9
Alternate Names
Calgranulin B, MRP-14
Gene Symbol
S100A9
UniProt
Additional S100A9 Products
Product Documents for S100A9 Antibody (S100A9/1075)
Product Specific Notices for S100A9 Antibody (S100A9/1075)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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