Have any of your S100A9 antibodies been tested for use in neutralization, or blocking assays, on human samples?

Unfortunately at this time we have not tested, or received customer feedback on our S100A9 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our S100A9 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional Innovators Reward Program information can be found using this link.

We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.

Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.

S100A9 Antibody [142Nd]

Name: S100A9 Antibody [142Nd]
Brand: Novus Biologicals
SKU: NBP1-18843

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-18843

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Product Datasheet / COA / SDS

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

142Nd

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all S100A9 Antibodies »

Product Specifications

Immunogen

This S100A9 antibody was developed against a peptide with sequence C-DTNADKQLSFEEF corresponding to internal region according to NP_002956.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

13 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for S100A9 Antibody [142Nd]

Western Blot: S100A9 Antibody [142Nd] [NBP1-18843]

Western Blot: S100A9 Antibody [NBP1-18843] - Staining of Human Bone Marrow (A) with antibody at 1 ug/mL and Gastrointestinal cancer (B) lysate with antibody at 0.5 ug/mL and negative control HepG2 (C) lysate. 5 ug protein in RIPA buffer. Detected by chemiluminescence.

Immunocytochemistry/ Immunofluorescence: S100A9 Antibody [142Nd] [NBP1-18843]

Immunocytochemistry/Immunofluorescence: S100A9 Antibody [NBP1-18843] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Immunocytochemistry/ Immunofluorescence: S100A9 Antibody [142Nd] [NBP1-18843]

Immunocytochemistry/Immunofluorescence: S100A9 Antibody [NBP1-18843] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Immunocytochemistry/Immunofluorescence: Goat Polyclonal S100A9 Antibody [NBP1-18843] -

Immunocytochemistry/Immunofluorescence: Goat Polyclonal S100A9 Antibody [NBP1-18843] - Immunofluorescence analysis of paraformaldehyde fixed THP-1 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Flow Cytometry: S100A9 Antibody [142Nd] [NBP1-18843]

Flow Cytometry: S100A9 Antibody [NBP1-18843] - Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunohistochemistry-Paraffin: Goat Polyclonal S100A9 Antibody [NBP1-18843] -

Immunohistochemistry-Paraffin: Goat Polyclonal S100A9 Antibody [NBP1-18843] - (7µg/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Control: Goat Polyclonal S100A9 Antibody [NBP1-18843] -

Control: Goat Polyclonal S100A9 Antibody [NBP1-18843] - Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.

Immunohistochemistry-Paraffin: S100A9 Antibody [142Nd] [NBP1-18843]

Immunohistochemistry-Paraffin: S100A9 Antibody [NBP1-18843] - Staining of paraffin embedded human lung. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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Applications for S100A9 Antibody [142Nd]

Application

Recommended Usage

ELISA

Detection limit 1:8000

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Western Blot

0.5 - 2 ug/mL

Application Notes

Approx 13-14kDa band observed in Human Bone Marrow lysates and approx 14-15kDa in Human Gastrointestinal cancer lysates (calculated MW of 13.2kDa according to NP_002956.1).

IHC: Paraffin embedded Human Lung. Recommended concentration: 2-4 ug/ml. Paraffin embedded Human Spleen. Recommended concentration: 6-7 ug/ml.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: S100A9

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. [provided by RefSeq]

Long Name

S100 Calcium Binding Protein A9

Alternate Names

Calgranulin B, MRP-14

Entrez Gene IDs

6280 (Human)

Gene Symbol

S100A9

UniProt

NP_002956.1 (Human)

Additional S100A9 Products

Product Documents for S100A9 Antibody [142Nd]

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for S100A9 Antibody [142Nd]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Flow Cytometry

S100A9 Antibody [142Nd]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-18843

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

Concentration

View all S100A9 Antibodies »

Product Specifications

Immunogen

Clonality

Host

Isotype

Theoretical MW

Scientific Data Images for S100A9 Antibody [142Nd]

Western Blot: S100A9 Antibody [142Nd] [NBP1-18843]

Immunocytochemistry/ Immunofluorescence: S100A9 Antibody [142Nd] [NBP1-18843]

Immunocytochemistry/ Immunofluorescence: S100A9 Antibody [142Nd] [NBP1-18843]

Immunocytochemistry/Immunofluorescence: Goat Polyclonal S100A9 Antibody [NBP1-18843] -

Flow Cytometry: S100A9 Antibody [142Nd] [NBP1-18843]

Immunohistochemistry-Paraffin: Goat Polyclonal S100A9 Antibody [NBP1-18843] -

Control: Goat Polyclonal S100A9 Antibody [NBP1-18843] -

Immunohistochemistry-Paraffin: S100A9 Antibody [142Nd] [NBP1-18843]

Applications for S100A9 Antibody [142Nd]

ELISA

Flow Cytometry

Immunocytochemistry/ Immunofluorescence

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: S100A9

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional S100A9 Products

Product Documents for S100A9 Antibody [142Nd]

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for S100A9 Antibody [142Nd]