SA1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-298
![Western Blot: SA1 Antibody [NB100-298]](https://resources.bio-techne.com/images/products/SA1-Antibody-Western-Blot-NB100-298-img0003.jpg "Western Blot: SA1 Antibody [NB100-298]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Invertebrate
Cited:
Invertebrate
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A 36 amino acid synthetic peptide, which represented a portion of human Stromal Antigen 1 encoded in part by exons 5 and 6 (LocusLink ID 10274).
Reactivity Notes
Invetebrate reactivity reported in (PMID: 23874961).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for SA1 Antibody - BSA Free
Western Blot: SA1 Antibody [NB100-298]
Western Blot: SA1 Antibody [NB100-298] - Nuclear extract from approx. 500,000 HeLa cells. Antibody used at 0.5 ug/ml.![Immunocytochemistry/ Immunofluorescence: SA1 Antibody [NB100-298]](https://resources.bio-techne.com/images/products/SA1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-298-img0002.jpg "Immunocytochemistry/ Immunofluorescence: SA1 Antibody [NB100-298]")
Immunocytochemistry/ Immunofluorescence: SA1 Antibody [NB100-298]
Immunocytochemistry/Immunofluorescence: SA1 Antibody [NB100-298] - HeLa cells that were extracted for 5 min at 4C in 0.5% Triton in CSK buffer. Antibody used at 1 ug/ml.Applications for SA1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:500-1:2000
Western Blot
1:1000-1:10000
Application Notes
NB 100-298 may be used for Western Blot where a band at ~145 kD is seen on SDS-PAGE. It may also be used for immunocytochemistry. Suggested working dilutions: * Western Blot 1:1,000-1:10,000 (ECL) Immunocytochemistry 1:500-1:2,000 Immunoprecipitation Not Done *The investigator should determine the optimal working dilution for a specific application.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: SA1
Alternate Names
cohesin subunit SA-1, nuclear protein stromal antigen 1, SA1, stromal antigen 1
Gene Symbol
STAG1
Additional SA1 Products
Product Documents for SA1 Antibody - BSA Free
Product Specific Notices for SA1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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