Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

1.0 mg/ml

View all SA1 Antibodies »

Product Summary for SA1 Antibody

Immunogen

A 35 amino acid synthetic peptide, which represented a portion of human Stromal Antigen 1 encoded in part by exons 35 and 36 (LocusLink ID 10274).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SA1 Antibody

Western Blot: SA1 Antibody [NB100-299]

Western Blot: SA1 Antibody [NB100-299]

Western Blot: SA1 Antibody [NB100-299] - Detection of human SA1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-SA1 antibody NB100-299 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunocytochemistry/ Immunofluorescence: SA1 Antibody [NB100-299]

Immunocytochemistry/ Immunofluorescence: SA1 Antibody [NB100-299]

Immunocytochemistry/Immunofluorescence: SA1 Antibody [NB100-299] - HeLa cells that were extracted for 5 min. at 4C in 0.5% Triton in CSK buffer. Antibody used at 1 ug/ml.
Western Blot: SA1 Antibody [NB100-299]

Western Blot: SA1 Antibody [NB100-299]

Western Blot: SA1 Antibody [NB100-299] - Nuclear extract from approx. 500,000 HeLa cells. Antibody used at 0.5 ug/ml.
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Applications for SA1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500-1:2000

Western Blot

1:1000-1:10000
Application Notes
NB 100-299 may be used for Western Blot where a band at ~145 kD is seen on SDS-PAGE. It may also be used for immunocytochemistry. *The investigator should determine the optimal working dilution for a specific application.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SA1

SA1 is a member of the SCC3 family and is expressed in the nucleus. It encodes a component of cohesin, a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase, after which it is dissociated in preparation for segregation during anaphase.

Alternate Names

cohesin subunit SA-1, nuclear protein stromal antigen 1, SA1, stromal antigen 1

Gene Symbol

STAG1

Additional SA1 Products

Product Documents for SA1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SA1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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