SA1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87097

Novus Biologicals, part of Bio-Techne
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NBP1-87097
NBP1-87097-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SA1 Antibodies »

Product Summary for SA1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TSELPVLQDSTNETTAHSDAGSELEETEVKGKRKRGRPGRPPSTNKKPRKSPGEKSR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SA1 Antibody

Western Blot: SA1 Antibody [NBP1-87097]

Western Blot: SA1 Antibody [NBP1-87097]

Western Blot: SA1 Antibody [NBP1-87097] - Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
SA1 Antibody Western Blot: SA1 Antibody [NBP1-87097] -

Western Blot: SA1 Antibody [NBP1-87097] -

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
SA1 Antibody Immunohistochemistry-Paraffin: SA1 Antibody [NBP1-87097] -

Immunohistochemistry-Paraffin: SA1 Antibody [NBP1-87097] -

Staining of human endometrium shows strong nuclear positivity in glandular cells.
View 3 more images

Applications for SA1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SA1

SA1 is a member of the SCC3 family and is expressed in the nucleus. It encodes a component of cohesin, a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase, after which it is dissociated in preparation for segregation during anaphase.

Alternate Names

cohesin subunit SA-1, nuclear protein stromal antigen 1, SA1, stromal antigen 1

Gene Symbol

STAG1

Additional SA1 Products

Product Documents for SA1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SA1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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