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SAGE1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84354

Novus Biologicals, part of Bio-Techne
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NBP1-84354
NBP1-84354-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HSVREEKMESGKPQTDKVISNDAPQLGHMAAGGIPSMSTKDLYATVTQNVHEERMENNQPQPSYDLSTVLPGLTYLTVAGIPAMSTRDQYATVTHNVHEEKIKNGQAASDNVFSTVPPAFINMAATGVSSMSTRDQYAAVTHNIR

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 26252478).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SAGE1 Antibody

Immunocytochemistry/ Immunofluorescence: SAGE1 Antibody [NBP1-84354]

Immunocytochemistry/ Immunofluorescence: SAGE1 Antibody [NBP1-84354]

Immunocytochemistry/Immunofluorescence: SAGE1 Antibody [NBP1-84354] - Staining of human cell line U-2 OS shows localization to nucleus.
Immunohistochemistry-Paraffin: SAGE1 Antibody [NBP1-84354]

Immunohistochemistry-Paraffin: SAGE1 Antibody [NBP1-84354]

Immunohistochemistry-Paraffin: SAGE1 Antibody [NBP1-84354] - Staining in human testis and endometrium tissues using anti-SAGE1 antibody. Corresponding SAGE1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SAGE1 Antibody [NBP1-84354]

Immunohistochemistry-Paraffin: SAGE1 Antibody [NBP1-84354]

Immunohistochemistry-Paraffin: SAGE1 Antibody [NBP1-84354] - Staining of human endometrium shows low expression as expected.

Applications for SAGE1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
IHC reported in scientific literature (PMID: 26252478). For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SAGE1

SAGE1 - sarcoma antigen 1

Alternate Names

putative tumor antigen, SAGECT14Cancer/testis antigen 14, sarcoma antigen 1

Gene Symbol

SAGE1

Additional SAGE1 Products

Product Documents for SAGE1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SAGE1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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