SALM4/LRFN3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14199

Novus Biologicals, part of Bio-Techne
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NBP2-14199
NBP2-14199-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SALM4/LRFN3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EATAAVELTVGPPPPPQLANSTSCDPPRDGDPDALTPPSAAS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SALM4/LRFN3 Antibody

Immunohistochemistry-Paraffin: SALM4/LRFN3 Antibody [NBP2-14199]

Immunohistochemistry-Paraffin: SALM4/LRFN3 Antibody [NBP2-14199]

Immunohistochemistry-Paraffin: LRFN3 Antibody [NBP2-14199] Staining of human hippocampus shows moderate cytoplasmic positivity in fraction of neuronal cells.

Applications for SALM4/LRFN3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SALM4/LRFN3

Synaptic adhesion-like molecule 4 (SALM4; also leucine-rich repeat and fibronectin type-III domain-containing protein 3 (Lrfn3) is an approximately 90 kDa member of the Lrfn family of type I transmembrane glycoproteins. Human SALM4 is synthesized as a 628 amino acid (aa) precursor that contains a 16 aa signal sequence, a 523 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 68 aa cytoplasmic region. The ECD consists of six leucine-rich repeats (LRR), an IgC2-like domain, and a fibronectin type-III domain, tandemly aligned in that order. In addition, there are five potential sites for N-linked glycosylation. SALM4 and SALM5 lack a C-terminal intracellular PDZ binding domain, which is conserved among SALMs 1-3. Mature human SALM4 shares 96% aa sequence identity with mature mouse SALM4. Northern blot analysis showed that in mice, SALM4 is strongly expressed in the adult brain and is also present in the adult gastrointestinal tract and kidneys. It is distributed throughout the neuron, including the growth cone. In the developing mouse embryo, a temporal expression profile blot revealed a general increment of expression around E10.5, with weak expression detected before E10.5. SALM4, like the other SALMs, promotes neurite outgrowth. Specifically, the SALMs modify total outgrowth and neurite branching.

Long Name

Leucine-rich Repeat and Fibronectin Type III Domain Containing Protein 3

Alternate Names

FIGLER1, LRFN3

Gene Symbol

LRFN3

Additional SALM4/LRFN3 Products

Product Documents for SALM4/LRFN3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SALM4/LRFN3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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