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SARS-CoV-2 nsp8 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07968

Novus Biologicals, part of Bio-Techne
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NBP3-07968

Key Product Details

Species Reactivity

SARS-CoV-2

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant SARS-CoV-2 NSP8(A3943-Q4118)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SARS-CoV-2 nsp8 Antibody - Azide and BSA Free

Western Blot: SARS-CoV-2 nsp8 AntibodyAzide and BSA Free [NBP3-07968]

Western Blot: SARS-CoV-2 nsp8 AntibodyAzide and BSA Free [NBP3-07968]

Western Blot: SARS-CoV-2 nsp8 Antibody [NBP3-07968] - Various lysates were subjected to SDS-PAGE followed by western blot with SARS-CoV-2 (2019-nCoV) NSP8 Protein antibody at dilution of 1:1000.

Applications for SARS-CoV-2 nsp8 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:4000-1:8000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, pH 7.4, 50% glycerol.

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: SARS-CoV-2 nsp8

SARS-CoV-2 Nonstructural Protein 8 (NSP8) is one of the sixteen nonstructural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). SARS-CoV-2 NSP8 is 198 amino acids (aa) with a theoretical molecular weight of 21.9 kDa (1,2). The amino acid sequence alignment of SARS-CoV and SARS-CoV-2's NSP8 has 97.5% sequence identity and 100% sequence similarity (1). SARS-CoV-2 NSP8 heterodimerizes with NSP7, which forms a complex with NSP12 (1-4). NSP8 alone in its monomeric form can also complex with NSP12, generating the RNA polymerase complex (1,4). Additionally, NSP8 is shown to interact with the SARS-CoV-2 open reading frame 6 (ORF6), encouraging RNA polymerase activity (1).

References

1. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4

2. Gordon, D. E., Jang, G. M., Bouhaddou, M., Xu, J., Obernier, K., White, K. M., O'Meara, M. J., Rezelj, V. V., Guo, J. Z., Swaney, D. L., Tummino, T. A., Huttenhain, R., Kaake, R. M., Richards, A. L., Tutuncuoglu, B., Foussard, H., Batra, J., Haas, K., Modak, M., Kim, M., ... Krogan, N. J. (2020). A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. https://doi.org/10.1038/s41586-020-2286-9

3. Qiu, Y., & Xu, K. (2020). Functional studies of the coronavirus nonstructural proteins. STEMedicine. https://doi.org/10.37175/stemedicine.v1i2.39

4. Peng, Q., Peng, R., Yuan, B., Zhao, J., Wang, M., Wang, X., Wang, Q., Sun, Y., Fan, Z., Qi, J., Gao, G. F., & Shi, Y. (2020). Structural and Biochemical Characterization of the nsp12-nsp7-nsp8 Core Polymerase Complex from SARS-CoV-2. Cell reports. https://doi.org/10.1016/j.celrep.2020.107774

Alternate Names

Non-structural protein 8, nsp8 replicase

Gene Symbol

ORF1ab

Additional SARS-CoV-2 nsp8 Products

Product Documents for SARS-CoV-2 nsp8 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SARS-CoV-2 nsp8 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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