SARS-CoV-2 nsp8 Products

SARS-CoV-2 Nonstructural Protein 8 (NSP8) is one of the sixteen nonstructural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). SARS-CoV-2 NSP8 is 198 amino acids (aa) with a theoretical molecular weight of 21.9 kDa (1,2). The amino acid sequence alignment of SARS-CoV and SARS-CoV-2's NSP8 has 97.5% sequence identity and 100% sequence similarity (1). SARS-CoV-2 NSP8 heterodimerizes with NSP7, which forms a complex with NSP12 (1-4). NSP8 alone in its monomeric form can also complex with NSP12, generating the RNA polymerase complex (1,4). Additionally, NSP8 is shown to interact with the SARS-CoV-2 open reading frame 6 (ORF6), encouraging RNA polymerase activity (1).

References

1. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4

2. Gordon, D. E., Jang, G. M., Bouhaddou, M., Xu, J., Obernier, K., White, K. M., O'Meara, M. J., Rezelj, V. V., Guo, J. Z., Swaney, D. L., Tummino, T. A., Huttenhain, R., Kaake, R. M., Richards, A. L., Tutuncuoglu, B., Foussard, H., Batra, J., Haas, K., Modak, M., Kim, M., ... Krogan, N. J. (2020). A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. https://doi.org/10.1038/s41586-020-2286-9

3. Qiu, Y., & Xu, K. (2020). Functional studies of the coronavirus nonstructural proteins. STEMedicine. https://doi.org/10.37175/stemedicine.v1i2.39

4. Peng, Q., Peng, R., Yuan, B., Zhao, J., Wang, M., Wang, X., Wang, Q., Sun, Y., Fan, Z., Qi, J., Gao, G. F., & Shi, Y. (2020). Structural and Biochemical Characterization of the nsp12-nsp7-nsp8 Core Polymerase Complex from SARS-CoV-2. Cell reports. https://doi.org/10.1016/j.celrep.2020.107774