SARS-CoV-2 nsp8 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15980
Key Product Details
Species Reactivity
SARS-CoV-2
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of coronavirus NSP8 (YP_009742615.1). AIASEFSSLPSYAAFATAQEAYEQAVANGDSEVVLKKLKKSLNVAKSEFDRDAAMQRKLEKMADQAMTQMYKQARSEDKRAKVTSAMQTMLFTMLRKLDNDALNNIINNARDGCVPLNIIPLTTAAKLMVVIPDYNTYKNTCDGTTFTYASALWEIQQVVDADSKIVQLSEISMDNSPNLAWPLIVTALRANSAVKLQ
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SARS-CoV-2 nsp8 Antibody - Azide and BSA Free
![Western Blot: SARS-CoV-2 nsp8 AntibodyAzide and BSA Free [NBP3-15980]](https://resources.bio-techne.com/images/products/SARS-CoV-2-nsp8-Antibody-Western-Blot-NBP3-15980-img0001.jpg "Western Blot: SARS-CoV-2 nsp8 AntibodyAzide and BSA Free [NBP3-15980]")
Western Blot: SARS-CoV-2 nsp8 AntibodyAzide and BSA Free [NBP3-15980]
Western Blot: SARS-CoV-2 nsp8 Antibody [NBP3-15980] - Western blot analysis of extracts of normal 293T cells and 293T transfected with NSP8 Protein, using SARS-CoV-2 nsp8 antibody (NBP3-15980) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.Applications for SARS-CoV-2 nsp8 Antibody - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:2000 - 1:10000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 50% glycerol, pH7.3
Format
Azide and BSA Free
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SARS-CoV-2 nsp8
References
1. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4
2. Gordon, D. E., Jang, G. M., Bouhaddou, M., Xu, J., Obernier, K., White, K. M., O'Meara, M. J., Rezelj, V. V., Guo, J. Z., Swaney, D. L., Tummino, T. A., Huttenhain, R., Kaake, R. M., Richards, A. L., Tutuncuoglu, B., Foussard, H., Batra, J., Haas, K., Modak, M., Kim, M., ... Krogan, N. J. (2020). A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. https://doi.org/10.1038/s41586-020-2286-9
3. Qiu, Y., & Xu, K. (2020). Functional studies of the coronavirus nonstructural proteins. STEMedicine. https://doi.org/10.37175/stemedicine.v1i2.39
4. Peng, Q., Peng, R., Yuan, B., Zhao, J., Wang, M., Wang, X., Wang, Q., Sun, Y., Fan, Z., Qi, J., Gao, G. F., & Shi, Y. (2020). Structural and Biochemical Characterization of the nsp12-nsp7-nsp8 Core Polymerase Complex from SARS-CoV-2. Cell reports. https://doi.org/10.1016/j.celrep.2020.107774
Alternate Names
Non-structural protein 8, nsp8 replicase
Gene Symbol
ORF1ab
Additional SARS-CoV-2 nsp8 Products
Product Documents for SARS-CoV-2 nsp8 Antibody - Azide and BSA Free
Product Specific Notices for SARS-CoV-2 nsp8 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov