SARS-CoV-2 ORF3a Antibody (HL1722) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25666
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
SARS-CoV-2
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1722
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for SARS-CoV-2 ORF3a Antibody (HL1722) - Azide and BSA Free
Immunogen
Recombinant protein encompassing a sequence within the center region of SARS-CoV-2 ORF3a. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SARS-CoV-2 ORF3a Antibody (HL1722) - Azide and BSA Free
Immunocytochemistry/Immunofluorescence: SARS-CoV-2 ORF3a Antibody (HL1722) - Azide and BSA Free [NBP3-25666] -
Immunocytochemistry/Immunofluorescence: SARS-CoV-2 ORF3a Antibody (HL1722) - Azide and BSA Free [NBP3-25666] - SARS-CoV-2 (COVID-19) ORF3a antibody [HL1722] detects SARS-CoV-2 (COVID-19) ORF3a protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) ORF3a stained by SARS-CoV-2 (COVID-19) ORF3a antibody [HL1722] (NBP3-25666) diluted at 1:500. Blue: Fluoroshield with DAPI .
Immunohistochemistry-Paraffin: SARS-CoV-2 ORF3a Antibody (HL1722) - Azide and BSA Free [NBP3-25666] -
Immunohistochemistry-Paraffin: SARS-CoV-2 ORF3a Antibody (HL1722) - Azide and BSA Free [NBP3-25666] - SARS-CoV-2 (COVID-19) ORF3a antibody [HL1722] detects SARS-CoV-2 (COVID-19) ORF3a protein by immunohistochemical analysis. Sample: Paraffin-embedded mock and SARS-CoV-2 (COVID-19) ORF3a transfected 293T. Green: SARS-CoV-2 (COVID-19) ORF3a stained by SARS-CoV-2 (COVID-19) ORF3a antibody [HL1722] (NBP3-25666) diluted at 1:1000. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: SARS-CoV-2 ORF3a Antibody (HL1722) - Azide and BSA Free [NBP3-25666] -
Western Blot: SARS-CoV-2 ORF3a Antibody (HL1722) - Azide and BSA Free [NBP3-25666] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) ORF3a antibody [HL1722] (NBP3-25666) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for SARS-CoV-2 ORF3a Antibody (HL1722) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SARS-CoV-2 ORF3a
The SARS-CoV-2 ORF3a protein encodes for a Ca2+ ion channel that functions in NLRP3 (NOD-, LRR- and pyrin domain-containing protein 3) inflammasome activation (3, 4). The ORF3a ion channel also functions in viral particle release as well as apoptotic and necrotic cell death (5). SARS-CoV-2 ORF3a interacts with the inflammasome component TRAF3 (TNF Receptor Associated Factor 3) which activates ASC (apoptosis-associated speck-like protein contain CARD) ubiquitination and, in-turn, stimulates caspase-1 activation and IL-1beta (interleukin-1-beta) production and maturation (3, 4). IL-1beta activation results in cytokine production and the overproduction of cytokines referred to as a cytokine storm, a hallmark of COVID-19 (4). Additionally, high throughput screening experiments have revealed the SARS-CoV-2 ORF3a binds to the human heme oxygenase (HMOX1) protein (5). HMOX1 has a role in reducing inflammation and tissue damage, both features of SARS-CoV-2 infection (5). The ORF3a-HMOX1 interaction will be worth exploring for its role in innate immune response and as a potential therapeutic target for COVID-19 (5).
Alternative names for SARS-CoV-2 ORF3a includes 2019-nCoV ORF3a protein, COVID-19 ORF3a, Human coronavirus ORF3a protein, ORF3a protein, SARS-CoV-2 accessory protein 3a, SARS-CoV-2 protein 3a, SARS-CoV-2 protein U274, and SARS-CoV-2 protein X1.
References
1. Michel, C. J., Mayenr, C., Poch, O., & Thompson, J. D. (2020). Characterization of accessory genes in coronavirus genomes. Virology journal. https://doi.org/10.1186/s12985-020-01402-12. UniProt (P0DTC3)
3. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The protein journal. https://doi.org/10.1007/s10930-020-09901-4
4. Oladele, J. O., Ajayi, E. I., Oyeleke, O. M., Oladele, O. T., Olowookere, B. D., Adeniyi, B. M., Oyewole, O. I., & Oladiji, A. T. (2020). A systematic review on COVID-19 pandemic with special emphasis on curative potentials of Nigeria based medicinal plants. Heliyon. https://doi.org/10.1016/j.heliyon.2020.e04897
5. Batra, N., De Souza, C., Batra, J., Raetz, A. G., & Yu, A. M. (2020). The HMOX1 Pathway as a Promising Target for the Treatment and Prevention of SARS-CoV-2 of 2019 (COVID-19). International journal of molecular sciences. https://doi.org/10.3390/ijms21176412
Alternate Names
2019-nCoV ORF3a, 2019-nCoV ORF3a Protein, COVID-19 ORF3a, Human coronavirus ORF3a Protein, ORF3a protein, SARS-CoV-2, SARS-CoV-2 Accessory protein 3a, SARSCoV2 ORF3a Protein, SARS-CoV-2 ORF3a Protein, SARS-CoV-2 Protein 3a, SARS-CoV-2 Protein U274, SARS-CoV-2 Protein X1, Severe Acute Respiratory Syndrome Coronavirus 2
Gene Symbol
ORF3a
Additional SARS-CoV-2 ORF3a Products
Product Documents for SARS-CoV-2 ORF3a Antibody (HL1722) - Azide and BSA Free
Product Specific Notices for SARS-CoV-2 ORF3a Antibody (HL1722) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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