SARS-CoV-2 ORF3a Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15985
Key Product Details
Species Reactivity
Validated:
SARS-CoV-2
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for SARS-CoV-2 ORF3a Antibody - Azide and BSA Free
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of coronavirus ORF3A (YP_009724391.1). GLEAPFLYLYALVYFLQSINFVRIIMRLWLCWKCRSKNPLLYDANYFLCWHTNCYDYCIPYNSVTSSIVITSGDGTTSPISEHDYQIGGYTEKWESGVKDC
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SARS-CoV-2 ORF3a Antibody - Azide and BSA Free
![Western Blot: SARS-CoV-2 ORF3a AntibodyAzide and BSA Free [NBP3-15985]](https://resources.bio-techne.com/images/products/SARS-CoV-2-ORF3a-Antibody-Western-Blot-NBP3-15985-img0001.jpg "Western Blot: SARS-CoV-2 ORF3a AntibodyAzide and BSA Free [NBP3-15985]")
Western Blot: SARS-CoV-2 ORF3a AntibodyAzide and BSA Free [NBP3-15985]
Western Blot: SARS-CoV-2 ORF3a Antibody [NBP3-15985] - Western blot analysis of extracts of 293T cells, using SARS-CoV-2 ORF3a antibody (NBP3-15985) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.Applications for SARS-CoV-2 ORF3a Antibody - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:2000 - 1:6000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 50% glycerol, pH7.3
Format
Azide and BSA Free
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SARS-CoV-2 ORF3a
The SARS-CoV-2 ORF3a protein encodes for a Ca2+ ion channel that functions in NLRP3 (NOD-, LRR- and pyrin domain-containing protein 3) inflammasome activation (3, 4). The ORF3a ion channel also functions in viral particle release as well as apoptotic and necrotic cell death (5). SARS-CoV-2 ORF3a interacts with the inflammasome component TRAF3 (TNF Receptor Associated Factor 3) which activates ASC (apoptosis-associated speck-like protein contain CARD) ubiquitination and, in-turn, stimulates caspase-1 activation and IL-1beta (interleukin-1-beta) production and maturation (3, 4). IL-1beta activation results in cytokine production and the overproduction of cytokines referred to as a cytokine storm, a hallmark of COVID-19 (4). Additionally, high throughput screening experiments have revealed the SARS-CoV-2 ORF3a binds to the human heme oxygenase (HMOX1) protein (5). HMOX1 has a role in reducing inflammation and tissue damage, both features of SARS-CoV-2 infection (5). The ORF3a-HMOX1 interaction will be worth exploring for its role in innate immune response and as a potential therapeutic target for COVID-19 (5).
Alternative names for SARS-CoV-2 ORF3a includes 2019-nCoV ORF3a protein, COVID-19 ORF3a, Human coronavirus ORF3a protein, ORF3a protein, SARS-CoV-2 accessory protein 3a, SARS-CoV-2 protein 3a, SARS-CoV-2 protein U274, and SARS-CoV-2 protein X1.
References
1. Michel, C. J., Mayenr, C., Poch, O., & Thompson, J. D. (2020). Characterization of accessory genes in coronavirus genomes. Virology journal. https://doi.org/10.1186/s12985-020-01402-12. UniProt (P0DTC3)
3. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The protein journal. https://doi.org/10.1007/s10930-020-09901-4
4. Oladele, J. O., Ajayi, E. I., Oyeleke, O. M., Oladele, O. T., Olowookere, B. D., Adeniyi, B. M., Oyewole, O. I., & Oladiji, A. T. (2020). A systematic review on COVID-19 pandemic with special emphasis on curative potentials of Nigeria based medicinal plants. Heliyon. https://doi.org/10.1016/j.heliyon.2020.e04897
5. Batra, N., De Souza, C., Batra, J., Raetz, A. G., & Yu, A. M. (2020). The HMOX1 Pathway as a Promising Target for the Treatment and Prevention of SARS-CoV-2 of 2019 (COVID-19). International journal of molecular sciences. https://doi.org/10.3390/ijms21176412
Alternate Names
2019-nCoV ORF3a, 2019-nCoV ORF3a Protein, COVID-19 ORF3a, Human coronavirus ORF3a Protein, ORF3a protein, SARS-CoV-2, SARS-CoV-2 Accessory protein 3a, SARSCoV2 ORF3a Protein, SARS-CoV-2 ORF3a Protein, SARS-CoV-2 Protein 3a, SARS-CoV-2 Protein U274, SARS-CoV-2 Protein X1, Severe Acute Respiratory Syndrome Coronavirus 2
Gene Symbol
ORF3a
Additional SARS-CoV-2 ORF3a Products
Product Documents for SARS-CoV-2 ORF3a Antibody - Azide and BSA Free
Product Specific Notices for SARS-CoV-2 ORF3a Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov