SARS-CoV-2 Spike S1 Antibody (SP185) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07065

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-07065
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Key Product Details

Species Reactivity

SARS-CoV-2

Applications

ELISA, Immunohistochemistry, Sandwich ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # SP185

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SARS-CoV-2 Spike S1 Antibodies »

Product Specifications

Immunogen

Recombinant SARS-CoV-2 Spike S1 protein

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

The antibody was expressed in HEK293F cells, and subsequently obtained from cell supernatant by protein A affinity chromatography.

Scientific Data Images for SARS-CoV-2 Spike S1 Antibody (SP185) - Azide and BSA Free

Sandwich ELISA: SARS-CoV-2 Spike S1 Antibody (SP185) [NBP3-07065] -

Sandwich ELISA: SARS-CoV-2 Spike S1 Antibody (SP185) [NBP3-07065] - Sandwich ELISA for SARS-CoV-2 Spike S1 Matched Pair Antibodies. Antibodies: SARS-CoV-2 (COVID-19) Spike Antibodies, NBP3-07065 and NBP3-26919. A sandwich ELISA was performed using SARS-CoV-2 Spike S1 antibody (NBP3-07065, 2ug/ml) as capture antibody, the Spike S1 recombinant protein as the binding protein, and the anti-SARS-CoV-2 Spike S1 antibody (NBP3-26919, 1ug/ml) as the detection antibody. Secondary: Mouse anti-human IgG HRP conjugate at 1:10000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 1.58 ng.
Sandwich ELISA: SARS-CoV-2 Spike S1 Antibody (SP185) [NBP3-07065] - Sandwich ELISA for SARS-CoV-2 Spike S1 Matched Pair Antibodies. A sandwich ELISA was performed using SARS-CoV-2 Spike S1 antibody (NBP3-07065, 2ug/ml) as capture antibody, a Spike S1 recombinant protein as the binding protein, and an anti-SARS-CoV-2 Spike S1 antibody (1ug/ml) as the detection antibody. Secondary: Streptavidin-HRP at 1:10000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 1.96 ng
SARS-CoV-2 Spike S1 Antibody (SP185) - Azide and BSA Free

Immunohistochemistry: SARS-CoV-2 Spike S1 Antibody (SP185) - Azide and BSA Free [NBP3-07065] -

Immunohistochemistry: SARS-CoV-2 Spike S1 Antibody (SP185) - Azide and BSA Free [NBP3-07065] - Validation of SARS-CoV-2 (COVID-19) Spike in COVID-19 Patient Lung.Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Spike antibody (3 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Spike protein was observed in the macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.
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Applications for SARS-CoV-2 Spike S1 Antibody (SP185) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS pH 7.2

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SARS-CoV-2 Spike S1

The SARS-CoV-2 Spike protein is one of the four major structural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1,2). The spike protein is the largest of the structural proteins, which also include the membrane (M), envelope (E), and nucleocapsid (N) proteins (1,2). The SARS-CoV-2 spike protein is a 1273 amino acid (aa) heterotrimeric class I fusion protein with each monomer having a theoretical molecular weight of approximately 180 kDa (1). The club-shaped spike protein contains several functional regions and domains including the S1 globular head region which contains the N-terminal receptor-binding domain (RBD) and the S2 stem region that contains the C-terminal fusion domain, two heptad regions, a transmembrane domain, and a cytoplasmic tail (1,2). The viral spike protein is critical for attachment of the virus with the host cell, resulting in fusion and virus entry into the cell (1,2). More specifically, the RBD of the spike protein is responsible for binding to the cell surface receptor angiotensin converting enzyme 2 (ACE2) (1,2). This spike-ACE2 interaction results in a conformational change permitting furin cleavage between the S1 and S2 domains and then cleavage at S2' by TMPRRS2, or another protease, allowing membrane fusion (1,2).

Given the critical role of the spike protein RBD in the interaction with the ACE2 receptor and viral entry, a number of neutralizing antibodies against the RBD have been developed as potential therapeutics for treating COVID-19 (3). These antibodies bind the RBD domain on the S1 subunit inhibiting the interaction with ACE2 (3). However, more studies need to be done as neutralizing antibodies can result in antibody-dependent enhancement, in which the viral entry and replication within the host cell is increased (4). One potential way to combat antibody-dependent enhancement is the use of nanobodies (4). Furthermore, there are currently several vaccine strategies that are in clinical trials, or recently federally approved, that utilize the spike protein in different forms (e.g. full length, S1 RBD, RBD-Fc, N-terminal) for protecting against SARS-CoV-2 infection (4,5). These vaccine strategies include DNA vaccines, viral vector-based vaccines, RNA vaccines, and subunit vaccines (4,5).

References

1. Pillay T. S. (2020). Gene of the month: the 2019-nCoV/SARS-CoV-2 novel coronavirus spike protein. Journal of Clinical Pathology. https://doi.org/10.1136/jclinpath-2020-206658

2. Malik Y. A. (2020). Properties of Coronavirus and SARS-CoV-2. The Malaysian Journal of Pathology.

3. Ho M. (2020). Perspectives on the development of neutralizing antibodies against SARS-CoV-2. Antibody Therapeutics. https://doi.org/10.1093/abt/tbaa009

4. Samrat, S. K., Tharappel, A. M., Li, Z., & Li, H. (2020). Prospect of SARS-CoV-2 spike protein: Potential role in vaccine and therapeutic development. Virus Research. https://doi.org/10.1016/j.virusres.2020.198141

5. Sternberg, A., & Naujokat, C. (2020). Structural features of coronavirus SARS-CoV-2 spike protein: Targets for vaccination. Life Sciences. https://doi.org/10.1016/j.lfs.2020.118056

Alternate Names

SARS-CoV-2

Gene Symbol

S

Additional SARS-CoV-2 Spike S1 Products

Product Documents for SARS-CoV-2 Spike S1 Antibody (SP185) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SARS-CoV-2 Spike S1 Antibody (SP185) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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