SARS Nucleocapsid Protein Antibody Pack

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05695

Novus Biologicals, part of Bio-Techne
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NBP3-05695
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Key Product Details

Species

SARS-CoV, SARS-CoV-2

Applications

Dual RNAscope ISH-IHC, ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Format

Sample-size Vials

Product Summary for SARS Nucleocapsid Protein Antibody Pack

This pack contains 1 vial each of: NBP2-44205-25ug (25 ug), NB100-56576SS (25 ug), NB100-56683SS (25 ug). NBP2-44205 is manufactured exclusively by Rockland Immunochemicals.

Product Specifications

Clonality

Polyclonal

Application Notes

See individual datasheets of components for their validated applications

Reactivity Notes

See individual datasheets of components for their validated species

Scientific Data Images for SARS Nucleocapsid Protein Antibody Pack

Immunohistochemistry: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695]

Immunohistochemistry: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695]

Immunohistochemistry: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Formalin-fixed paraffin-embedded tissue sections of SARS-CoV-2 infected human lung tissue was processed for immunohistochemistry using rabbit polyclonal anti-SARS Nucleocapsid Antibody [NB100-56576] at 15ug/mL with 1 hr incubation at 25 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells.
Immunocytochemistry/ Immunofluorescence: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695]

Immunocytochemistry/ Immunofluorescence: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695]

Immunocytochemistry/Immunofluorescence: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody[NBP2-44205], showing viral protein synthesis. VeroE6 cells were infected with rSARS-CoV-2. Mock: non-infected cells. At 48 h.p.i, cells were fixed and prepared for immunofluorescence staining with primary antibodies directed against double-stranded RNA (dsRNA) [green] and SARS-CoV Nucleocapsid (N) [red]. Nuclear Counterstain DAPI [blue].
Western Blot: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695]

Western Blot: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695]

Western Blot: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Analysis of SARS Nucleocapsid in (A) untransfected mouse melanoma cell lysate and (B) transfected cell lysate using this antibody [NB100-56683] at a 1:2000 dilution.
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Kit Contents for SARS Nucleocapsid Protein Antibody Pack

Formulation, Preparation, and Storage

Formulation

NB100-56576 and NB100-56683: PBS, 0.05% Sodium Azide. NBP2-44205: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% Sodium Azide.

Concentration

Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SARS Nucleocapsid Protein

It has recently been shown that SARS is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139 kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics.

Alternate Names

N, N protein, N structural protein, NC, Nucleocapsid protein, Nucleoprotein, Protein N, SARS coronavirus N protein, SARS coronavirus nucleocapsid protein, SARS CoV, SARS CoV N protein, SARS CoV nucleocapsid protein, SARS N protein, SARS Nucleoprotein, SARSCoV, SARSCoV N protein, SARSCoV nucleocapsid protein, Severe acute respiratory syndrome, 2019-nCoV Nucleocapsid Protein, COVID-19 N protein, COVID-19 nucleocapsid protein, SARSCOV2 N protein, SARS-CoV-2 N protein, SARS-COV-2 nucleocapsid protein, SARS-COV-2 Nucleoprotein

Gene Symbol

N

Additional SARS Nucleocapsid Protein Products

Product Documents for SARS Nucleocapsid Protein Antibody Pack

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SARS Nucleocapsid Protein Antibody Pack

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

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