SARS Nucleocapsid Protein Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56576
Conjugate
Catalog #
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
SARS-CoV, SARS-CoV-2, Virus
Cited:
Human, Hamster, SARS-CoV, Viral, Virus, Virus - SARS-CoV-2
Applications
Validated:
Dual RNAscope ISH-IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, SDS-Page, Western Blot
Cited:
Dual RNAscope ISH-IHC, Electron Microscopy, ELISA, Enzyme-linked Immunosorbent Spot, IF/ICC, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, In Situ Hybridization, In vivo assay, Mass Spectrometry, RNAscope compatible, SDS-Page, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The antibody was developed by immunizing Rabbit with a synthetic peptide corresponding to amino acids 399-411 (DLDDFSKQLQQSM-C) from the N (SARS Nucleocapsid) for the Human SARS coronavirus (Genbank accession no. YP_009724397.2)
Reactivity Notes
Use in SARS-CoV-2 reported in scientific literature (PMID:33807059).
Specificity
Dot Blot results using recombinant proteins for cross-reactivity testing revealed high reactivity to SARS-CoV-2 Nucleocapsid protein (NBP2-90975) and low/no reactivity towards H1N1 (NBP1-99041). No cross-reactivity observed with influenza A(H1N1) virus, influenza B virus, respiratory syncytial virus, parainfluenza virus type 3, human coronavirus (HCoV) 229E, or MERS-CoV in PCR-confirmed tissue samples .
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SARS Nucleocapsid Protein Antibody - BSA Free
Immunohistochemistry: SARS Nucleocapsid Protein Antibody - BSA Free [NB100-56576]
Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NB100-56576] - Immunostaining of severe acute respiratory syndrome coronavirus 2 in pulmonary tissues from fatal coronavirus disease cases. A) P5 (Patient 5): scattered immunostaining of tracheal epithelial cells. B) P5: higher magnification shows immunostaining of ciliated cells. C) P8: immunostaining of desquamated type I pneumocyte in an alveolar lumen. D) P4: colocalization of SARS-CoV-2 viral antigen (red) with type II pneumocyte stained by surfactant (brown; arrow). E) P4: colocalization of SARS-CoV-2 viral antigen (red) with macrophages stained by CD163 (brown; arrows); virus immunostaining within type II pneumocytes is also seen (arrowheads). F) P4: extensive immunostaining of hyaline membranes in a region of exudative DAD. G) P3: scattered immunostaining within macrophage in hilar lymph node; anthracosis is also present. Emerg Infect Dis. 2020 May 21;26(9) 10.3201/eid2609.202095, PMID: 32437316Immunohistochemistry: SARS Nucleocapsid Protein Antibody - BSA Free [NB100-56576]
Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NB100-56576] - Pathological changes in rhesus macaques infected with SARS-CoV-2. (g) SARSCoV-2 antigen is detected by immunohistochemistry in type I pneumocytes. Magnification 400x. (j) SARS-CoV-2 antigen is detected by immunohistochemistry in type I pneumocytes (asterisk) and type II pneumocytes (arrow) as well as alveolar macrophages (arrowheads). Magnification 400x. (k) SARS-CoV-2 antigen is detected by immunohistochemistry in mediastinal lymph node. Magnification 400x. (l) SARSCoV-2 antigen is detected by immunohistochemistry in macrophages and lymphocytes in the lamina propria of the cecum. Magnification 400x. bioRxiv March 21, 2020 https://doi.org/10.1101/2020.03.21.001628
Dual RNAscope ISH-IHC: SARS Nucleocapsid Protein Antibody [NB100-56576] - Formalin-fixed paraffin-embedded tissue sections of SARS-CoV-2 infected human lung tissue were probed for SARS-CoV-2 viral RNA (ACD anti-sense specific probe v-nCoV2019-S (848561); Fast Red chromogen, ACD [322360]). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal anti-SARS Nucleocapsid Antibody [NB100-56576] at 15ug/mL with 1 hr incubation at 25 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells.
Applications for SARS Nucleocapsid Protein Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:500. Use reported in scientific literature (Mossel et al (2008))
Immunohistochemistry
reported in scientific literature (PMID 32396922)
Immunohistochemistry-Paraffin
reported in scientific literature (PMID 24725942)
SDS-Page
reported in scientific literature (PMID 34880383)
Western Blot
1:100-1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SARS Nucleocapsid Protein
Alternate Names
N, N protein, N structural protein, NC, Nucleocapsid protein, Nucleoprotein, Protein N, SARS coronavirus N protein, SARS coronavirus nucleocapsid protein, SARS CoV, SARS CoV N protein, SARS CoV nucleocapsid protein, SARS N protein, SARS Nucleoprotein, SARSCoV, SARSCoV N protein, SARSCoV nucleocapsid protein, Severe acute respiratory syndrome, 2019-nCoV Nucleocapsid Protein, COVID-19 N protein, SARS-CoV-2 N protein
Gene Symbol
N
Additional SARS Nucleocapsid Protein Products
Product Documents for SARS Nucleocapsid Protein Antibody - BSA Free
Product Specific Notices for SARS Nucleocapsid Protein Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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