SARS Spike Protein Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56048

Novus Biologicals, part of Bio-Techne
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NB100-56048
NB100-56048SS
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Key Product Details

Species Reactivity

SARS-CoV

Applications

Validated:

ELISA, In vitro assay, Western Blot

Cited:

In vitro assay, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all SARS Spike Protein Antibodies »

Product Specifications

Immunogen

The antibody was developed by immunizing rabbits with a synthetic peptide corresponding to amino acids 288-303 (AVDCALDPLSETKCTL) from the S (Spike glycoprotein)(Spike protein 1) for the Human SARS coronavirus (Genbank accession no. YP_009724390)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: COVID-19 (69%)

Specificity

The antibody was tested on a cell line transfected with a partial-length cDNA coding for the N-terminal portion of SARS Spike protein with a predicted molecular weight of 68 kDa.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SARS Spike Protein Antibody - BSA Free

Western Blot: SARS Spike Protein Antibody [NB100-56048]

Western Blot: SARS Spike Protein Antibody [NB100-56048]

Western Blot: Sars Spike Protein Antibody [NB100-56048] - Used at 1 ug/ml to analyze SARS Spike in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate.

Applications for SARS Spike Protein Antibody - BSA Free

Application
Recommended Usage

ELISA

0.1-1 ug/ml

In vitro assay

reported in scientific literature (PMID 34120764)

Western Blot

0.1-1 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.2% gelatin

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SARS Spike Protein

It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosilated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.

Alternate Names

E2, E2 glycoprotein, Human coronavirus spike glycoprotein, Peplomer protein, S, S glycoprotein, SARS coronavirus S protein, SARS coronavirus Spike Protein, SARS CoV, SARS CoV S glycoprotein, SARS CoV Spike protein, SARSCoV, SARS-CoV S protein, SARS-COV Spike, Severe acute respiratory syndrome spike glycoprotein, Severe acute respiratory syndrome virus spike glycoprotein, Spike glycoprotein, VGL2

Additional SARS Spike Protein Products

Product Documents for SARS Spike Protein Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SARS Spike Protein Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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