SART1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89023

Novus Biologicals, part of Bio-Techne
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NBP1-89023
NBP1-89023-25ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SART1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RRVKKIRKKEKEVVVRADDLLPLGDQTQDGDFGSRLRGRGRRRVSEVEEEKEPVPQPLPSDDTRVENMDISDEEEGGAPPPGSPQVLEEDEAELELQKQLEKG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SART1 Antibody

Western Blot: SART1 Antibody [NBP1-89023]

Western Blot: SART1 Antibody [NBP1-89023]

Western Blot: SART1 Antibody [NBP1-89023] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG.
Immunocytochemistry/ Immunofluorescence: SART1 Antibody [NBP1-89023]

Immunocytochemistry/ Immunofluorescence: SART1 Antibody [NBP1-89023]

Immunocytochemistry/Immunofluorescence: SART1 Antibody [NBP1-89023] - Staining of human cell line A-431 shows localization to nuclear speckles & the Golgi apparatus.
Immunohistochemistry-Paraffin: SART1 Antibody [NBP1-89023]

Immunohistochemistry-Paraffin: SART1 Antibody [NBP1-89023]

Immunohistochemistry-Paraffin: SART1 Antibody [NBP1-89023] - Staining of human stomach shows strong nuclear positivity in glandular cells.
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Applications for SART1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SART1

SART1, also known as hypoxia-associated factor (HAF), encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full-length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor-rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri-snRNP to the pre-spliceosome in the spliceosome assembly pathway. [provided by RefSeq]

Long Name

Squamous cell carcinoma antigen recognized by T cells

Alternate Names

Ara1, HAF, HOMS1, IgE Autoantigen, SART1259, SNRNP110, Snu66

Gene Symbol

SART1

Additional SART1 Products

Product Documents for SART1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SART1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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