SART1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14836

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (94%). Backed by our 100% Guarantee.

Applications

ICC/IF (Negative), Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.3 mg/ml

View all SART1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a internal portion of the human SART1 protein (between residues 100-200) [UniProt O43290]

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: rat (94%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SART1 Antibody - BSA Free

Western Blot: SART1 AntibodyBSA Free [NBP2-14836]

Western Blot: SART1 AntibodyBSA Free [NBP2-14836]

Western Blot: SART1 Antibody [NBP2-14836] - SART1 antibody tested in NIH-3T3 cell lysate.
Immunohistochemistry-Paraffin: SART1 Antibody - BSA Free [NBP2-14836]

Immunohistochemistry-Paraffin: SART1 Antibody - BSA Free [NBP2-14836]

Immunohistochemistry-Paraffin: SART1 Antibody [NBP2-14836] - SART1 antibody was tested in mouse testes using DAB with hematoxylin counterstain.

Applications for SART1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200-1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

1 ug/ml
Application Notes
This SART1 antibody is useful for IHC-paraffin embedded sections and Western blot. In Western blot a band is detected ~100 kDa in NIH-3T3 cell lysate. In IHC-P, staining was observed in the nuclei of mouse testes. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SART1

SART1 (HAF, Ara1, HOMS1, Snu66) is part of the SNU66/SART1 family. This protein is subject to phosphorylation, and sumoylation by SUMO2. It is highly expressed in the nucleus of highly proliferative cells, but is also expressed in the cytosol of epithelial cancers. HAF is a transcription factor that enhances hypoxia inducible factor 1 (HIF-1)-induced activation in VEGF and EPO genes under hypoxic conditions (http://www.aacrmeetingabstracts.org/cgi/content/abstract/2006/1/409-d). SART1/HAF is up-regulated in a multitude of tumors. During long periods of hypoxia, its level increases, but the levels are decreased during acute hypoxia. A recent study showed that SART1/HAF is an E3 ubiquitin ligase that binds and ubiquitinates HIF-1 alpha by an oxygen and pVHL-independent mechanism, which then targets HIF-1 alpha for proteasomal degradation. SART1/HAF also binds HIF-2 alpha at a separate site and increases HIF-2 alpha transactivation without triggering degradation. Therefore, HAF is able to alter the response of cancer cells from HIF-1 alpha-dependent to HIF-2 alpha-dependent transcription and initiates genes involved in invasion such as MMP9, PAI-1, and the stem cell factor OCT-3/4. The shift to HIF-2 alpha dependent gene expression instigated by HAF also promotes an enhanced tumor stem cell population, which results in extremely aggressive tumors in vivo. SART1/HAF presents a method for more aggressive tumor growth under sustained hypoxia (PMID: 21512133; 18838541; 19377289)

Long Name

Squamous cell carcinoma antigen recognized by T cells

Alternate Names

Ara1, HAF, HOMS1, IgE Autoantigen, SART1259, SNRNP110, Snu66

Entrez Gene IDs

9092 (Human); 20227 (Mouse)

Gene Symbol

SART1

UniProt

O43290, Q9Z315 (Mouse)

Additional SART1 Products

Product Documents for SART1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SART1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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