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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2B7

Concentration

1 mg/ml

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 167-383 of human SAV1 (NP_068590) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SAV1 Antibody (OTI2B7)

Western Blot: SAV1 Antibody (OTI2B7) [NBP2-45687]

Western Blot: SAV1 Antibody (OTI2B7) [NBP2-45687]

Western Blot: SAV1 Antibody (2B7) [NBP2-45687] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAV1 .
Immunohistochemistry: SAV1 Antibody (OTI2B7) [NBP2-45687]

Immunohistochemistry: SAV1 Antibody (OTI2B7) [NBP2-45687]

Immunohistochemistry: SAV1 Antibody (2B7) [NBP2-45687] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0) Dilution: 1:150

Applications for SAV1 Antibody (OTI2B7)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000
Application Notes
For immunohistochemistry in formalin or paraformaldyhyde fixed tissues, we recommend Heat-induced epitope retrieval by Tris-EDTA, pH8.0.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SAV1

WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 2 WW domains and a coiled-coil region. It is ubiquitously expressed in adult tissues. The encoded protein is 94% identical to the mouse protein at the amino acid level.

Alternate Names

45 kDa WW domain protein, hWW45, protein salvador homolog 1, salvador homolog 1 (Drosophila), SAV, WW domain-containing, WW45salvador, WWP4,1700040G09Rik

Gene Symbol

SAV1

Additional SAV1 Products

Product Documents for SAV1 Antibody (OTI2B7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SAV1 Antibody (OTI2B7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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