Scaffold attachment factor B2 Antibody (4H3)

Novus Biologicals, part of Bio-Techne | Catalog # H00009667-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4H3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Scaffold attachment factor B2 Antibodies »

Product Specifications

Immunogen

SAFB2 (NP_055464, 103 a.a. ~ 199 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NGLEDDSRDGQEDMEASLENLQNMGMMDMSVLDETEVANSSAPDFGEDGTDGLLDSFCDSKEYVAAQLRQLPAQPPEHAVDGEGFKNTLETSSLNFK

Specificity

SAFB2 - scaffold attachment factor B2 (4H3)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Scaffold attachment factor B2 Antibody (4H3)

Western Blot: Scaffold attachment factor B2 Antibody (4H3) [H00009667-M01]

Western Blot: Scaffold attachment factor B2 Antibody (4H3) [H00009667-M01]

Western Blot: Scaffold attachment factor B2 Antibody (4H3) [H00009667-M01] - Analysis of SAFB2 expression in transfected 293T cell line by SAFB2 monoclonal antibody (M01), clone 4H3.Lane 1: SAFB2 transfected lysate (Predicted MW: 57.2 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: Scaffold attachment factor B2 Antibody (4H3) [H00009667-M01] - Detection limit for recombinant GST tagged SAFB2 is approximately 3ng/ml as a capture antibody.

Applications for Scaffold attachment factor B2 Antibody (4H3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Scaffold attachment factor B2

Scaffold attachment factor B2 (SAFB2) is a chromatin associated protein that interacts with DNA elements termed S/MARs (scaffold or matrix attachment regions) but is not part of the nuclear matrix. SAFB2 is structurally and functionally similar to SAFB1 and appears to be a multifunctional protein involved in chromatin organization, mRNA processing and transcriptional regulation. SAFB2 has been demonstrated to interact with and function as a corepressor with many nuclear receptors such as estrogen receptor-alpha, PPAR-gamma, and FXR-alpha.

Alternate Names

KIAA0138SAF-B2, scaffold attachment factor B2

Gene Symbol

SAFB2

UniProt

NP_055464 (Human)

Additional Scaffold attachment factor B2 Products

Product Documents for Scaffold attachment factor B2 Antibody (4H3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Scaffold attachment factor B2 Antibody (4H3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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