SCAP2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB110-40659
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-GLVPKAYIMEMYDI corresponding to C-Terminus according to NP_003921.2.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for SCAP2 Antibody
![Western Blot: SCAP2 Antibody [NB110-40659]](https://resources.bio-techne.com/images/products/SCAP2-Antibody-Western-Blot-NB110-40659-img0003.jpg "Western Blot: SCAP2 Antibody [NB110-40659]")
Western Blot: SCAP2 Antibody [NB110-40659]
Western Blot: SCAP2 Antibody [NB110-40659] - Analysis of SCAP2 in Jurkat lysate (35ug protein in RIPA buffer) using this antibody at 0.5ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.![Western Blot: SCAP2 Antibody [NB110-40659]](https://resources.bio-techne.com/images/products/SCAP2-Antibody-Western-Blot-NB110-40659-img0002.jpg "Western Blot: SCAP2 Antibody [NB110-40659]")
Western Blot: SCAP2 Antibody [NB110-40659]
Western Blot: SCAP2 Antibody [NB110-40659] - HEK293 overexpressing SKAP2 and probed with (mock transfection in first lane).Applications for SCAP2 Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:32000
Western Blot
0.5 - 2 ug/ml
Application Notes
WB: Approx. 38 kDa band observed in Jurkat lysates (calculated MW of 41.2 kDa band according to NP_003921.2). In transfected HEK293 transiently expressing SKAP2 a band of approx. 45 kDa is observed. This band is not observed in the non-transfected HEK293. The calculated
An additional band of unknown identity was also consistently observed in Jurkat at 28 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SCAP2
Alternate Names
Fyn-associated phosphoprotein SKAP55 homologue, PRAP, RA70Pyk2/RAFTK-associated protein, Retinoic acid-induced protein 70, SAPSSKAP55 homolog, SCAP2MGC33304, SKAP55RSKAP-55HOM, SKAP-HOMMGC10411, src family associated phosphoprotein 2, Src family-associated phosphoprotein 2, src kinase associated phosphoprotein 2, src kinase-associated phosphoprotein 2, Src kinase-associated phosphoprotein 55-related protein, src kinase-associated phosphoprotein of 55-related protein, Src-associated adapter protein with PH and SH3 domains, src-associated adaptor protein
Entrez Gene IDs
8935 (Human)
Gene Symbol
SKAP2
UniProt
Additional SCAP2 Products
Product Documents for SCAP2 Antibody
Product Specific Notices for SCAP2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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