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SCAP2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47289

Novus Biologicals, part of Bio-Techne
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NBP2-47289
NBP2-47289-25ul

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for SCAP2 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LPEEIRNLLADVETFVADILKGENLSKKAKEKRESLIKKIKDVKSIYLQEFQDKGDAEDGEEYDDPFAGPPDTISLASERYDKDDEAPSDGAQFPPIAAQDLPFVLKAGYLEKRRKDHSFLGFEWQKRWCALSKTVFYY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SCAP2 Antibody

Western Blot: SCAP2 Antibody [NBP2-47289]

Western Blot: SCAP2 Antibody [NBP2-47289]

Western Blot: SCAP2 Antibody [NBP2-47289] - Analysis in control (vector only transfected HEK293T lysate) and SKAP2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: SCAP2 Antibody [NBP2-47289]

Immunocytochemistry/ Immunofluorescence: SCAP2 Antibody [NBP2-47289]

Immunocytochemistry/Immunofluorescence: SCAP2 Antibody [NBP2-47289] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: SCAP2 Antibody [NBP2-47289]

Immunohistochemistry-Paraffin: SCAP2 Antibody [NBP2-47289]

Immunohistochemistry-Paraffin: SCAP2 Antibody [NBP2-47289] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Applications for SCAP2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SCAP2

SCAP2 is encoded by this gene belongs to the src family kinases. This protein is similar to the src kinase associated phosphoprotein 1. It is an adaptor protein that is thought to play an essential role in the src signaling pathway in various cells. It inhibits PTK2B/RAFTK activity and regulates alpha-synuclein phosphorylation.

Alternate Names

Fyn-associated phosphoprotein SKAP55 homologue, PRAP, RA70Pyk2/RAFTK-associated protein, Retinoic acid-induced protein 70, SAPSSKAP55 homolog, SCAP2MGC33304, SKAP55RSKAP-55HOM, SKAP-HOMMGC10411, src family associated phosphoprotein 2, Src family-associated phosphoprotein 2, src kinase associated phosphoprotein 2, src kinase-associated phosphoprotein 2, Src kinase-associated phosphoprotein 55-related protein, src kinase-associated phosphoprotein of 55-related protein, Src-associated adapter protein with PH and SH3 domains, src-associated adaptor protein

Gene Symbol

SKAP2

Additional SCAP2 Products

Product Documents for SCAP2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SCAP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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