SCAP2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47289
Key Product Details
Validated by
Orthogonal Validation, Independent Antibodies
Species Reactivity
Validated:
Human
Predicted:
Mouse (94%), Rat (91%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for SCAP2 Antibody
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: LPEEIRNLLADVETFVADILKGENLSKKAKEKRESLIKKIKDVKSIYLQEFQDKGDAEDGEEYDDPFAGPPDTISLASERYDKDDEAPSDGAQFPPIAAQDLPFVLKAGYLEKRRKDHSFLGFEWQKRWCALSKTVFYY
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SCAP2 Antibody
Western Blot: SCAP2 Antibody [NBP2-47289]
Western Blot: SCAP2 Antibody [NBP2-47289] - Analysis in control (vector only transfected HEK293T lysate) and SKAP2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunocytochemistry/ Immunofluorescence: SCAP2 Antibody [NBP2-47289]
Immunocytochemistry/Immunofluorescence: SCAP2 Antibody [NBP2-47289] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.Immunohistochemistry-Paraffin: SCAP2 Antibody [NBP2-47289]
Immunohistochemistry-Paraffin: SCAP2 Antibody [NBP2-47289] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.Applications for SCAP2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SCAP2
Alternate Names
Fyn-associated phosphoprotein SKAP55 homologue, PRAP, RA70Pyk2/RAFTK-associated protein, Retinoic acid-induced protein 70, SAPSSKAP55 homolog, SCAP2MGC33304, SKAP55RSKAP-55HOM, SKAP-HOMMGC10411, src family associated phosphoprotein 2, Src family-associated phosphoprotein 2, src kinase associated phosphoprotein 2, src kinase-associated phosphoprotein 2, Src kinase-associated phosphoprotein 55-related protein, src kinase-associated phosphoprotein of 55-related protein, Src-associated adapter protein with PH and SH3 domains, src-associated adaptor protein
Gene Symbol
SKAP2
Additional SCAP2 Products
Product Documents for SCAP2 Antibody
Product Specific Notices for SCAP2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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