Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all SCC112 Antibodies »

Product Specifications

Immunogen

A synthetic peptide mapping to a region between residues 1250 and 1297 (C-terminus) of human SCC-112 using the numbering given in entry NP_056015.1 (GeneID 23244).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SCC112 Antibody

Western Blot: SCC112 Antibody [NB100-426]

Western Blot: SCC112 Antibody [NB100-426]

Western Blot: SCC112 Antibody [NB100-426] - Samples : Nuclear extract (5 and 20 ug for WB, 1 mg for IP) from HeLa cells. Antibodies : Affinity purified rabbit anti-SCC-112 antibody used at 0.1 ug/ml for WB and at 2 ug/mg extract for IP. Immunoprecipitated SCC-112 was detected with affinity purified rabbit anti-SCC-112 antibody used at 0.06 ug/ml. Detection : Chemiluminescence with an exposure time of less than 5 minutes.
Western Blot: SCC112 Antibody [NB100-426]

Western Blot: SCC112 Antibody [NB100-426]

Western Blot: SCC112 Antibody [NB100-426] - Detection of human SCC-112 in Western Blot and immunoprecipitation, using NB100-426. Samples: HeLa nuclear extracts. Exposure time of less than 5 minutes.
Western Blot: SCC112 Antibody [NB100-426]

Western Blot: SCC112 Antibody [NB100-426]

Western Blot: SCC112 Antibody [NB100-426] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SCC-112 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for SCC112 Antibody

Application
Recommended Usage

Immunoprecipitation

1-4 ug/mg of lysate

Western Blot

1:10000-1:25000
Application Notes
Western blot and immunoprecipitation. Immunohistochemistry has not been tested. *The investigator should determine the optimal working dilution for a specific application.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SCC112

SCC-112 is a novel cell cycle-regulated molecule. It is a nuclear protein of ~147 kDa, suggested to be associated with normal cell growth and proliferation.

Alternate Names

Cell proliferation-inducing gene 54 protein, DKFZp686B19246, FLJ41012, KIAA0648MGC131948, PDS5, PDS5, regulator of cohesion maintenance, homolog A (S. cerevisiae), PIG54, SCC-112MGC161503, Sister chromatid cohesion protein 112, sister chromatid cohesion protein PDS5 homolog A

Entrez Gene IDs

23244 (Human)

Gene Symbol

PDS5A

Additional SCC112 Products

Product Documents for SCC112 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SCC112 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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