SCP3/SYCP3 Antibody (PSH02-24)

Western Blot: SCP3/SYCP3 Antibody (PSH02-24) [NBP3-32934]

Western Blot: SCP3/SYCP3 Antibody (PSH02-24) [NBP3-32934] - Western blot analysis of SCP3/SYCP3 on SCP3 recombinant protein with Rabbit anti-SCP3/SYCP3 antibody (NBP3-32934) at 1/1,000 dilution.

Lysates/proteins at 30 ng/Lane.

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32934) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: SCP3/SYCP3 Antibody (PSH02-24) [NBP3-32934]

Immunohistochemistry: SCP3/SYCP3 Antibody (PSH02-24) [NBP3-32934] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-SCP3/SYCP3 antibody (NBP3-32934) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32934) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.