SDHAF4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86324
Key Product Details
Species Reactivity
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SPLLCHSLRKTSSSQGGKSELVKQSLKKPKLPEGRFDAPEDSHLEKEPLEKFPDDVNPVTKEKGGPRGPEPTRYGDWERKGRCID
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SDHAF4 Antibody
Western Blot: SDHAF4 Antibody [NBP1-86324]
Western Blot: SDHAF4 Antibody [NBP1-86324] - Analysis in control (vector only transfected HEK293T lysate) and C6orf57 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunocytochemistry/ Immunofluorescence: SDHAF4 Antibody [NBP1-86324]
Immunocytochemistry/Immunofluorescence: SDHAF4 Antibody [NBP1-86324] - Staining of human cell line A-431 shows localization to nucleus & mitochondria. Antibody staining is shown in green.Immunohistochemistry-Paraffin: SDHAF4 Antibody [NBP1-86324]
Immunohistochemistry-Paraffin: SDHAF4 Antibody [NBP1-86324] - Staining of human prostate shows weak to moderate cytoplasmic positivity in glandular cells.Applications for SDHAF4 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SDHAF4
Alternate Names
chromosome 6 open reading frame 57, hypothetical protein LOC135154, MGC104225
Gene Symbol
C6ORF57
Additional SDHAF4 Products
Product Documents for SDHAF4 Antibody
Product Specific Notices for SDHAF4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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