SDHB Antibody (SDHB/2126) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08809
![Western Blot: SDHB Antibody (SDHB/2126)Azide and BSA Free [NBP3-08809]](https://resources.bio-techne.com/images/products/SDHB-Antibody-SDHB-2126-Azide-and-BSA-Free-Western-Blot-NBP3-08809-img0002.jpg "Western Blot: SDHB Antibody (SDHB/2126)Azide and BSA Free [NBP3-08809]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SDHB/2126
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for SDHB Antibody (SDHB/2126) - Azide and BSA Free
Immunogen
Recombinant human SDHB fragment around aa 165-273 (Exact sequence is proprietary) (Uniprot: P21912 )
Reactivity Notes
Others not known.
Localization
Cytoplasmic (Mitochondria)
Specificity
Succinate dehydrogenase (SDH) is Complex II in the mitochondria, vital for mitochondrial electron transport, as well as Krebs cycle function. Four subunits comprise the SDH protein complex: a flavochrome subunit (SDHA), an iron-sulfur protein (SDHB) and two membrane-bound subunits (SDHC and SDHD) anchored to the inner mitochondrial membrane. The SDH complex functions as a tumor suppressor. Loss of any subunit proteins lead to destabilization of the complex and tumor formation. Antibody to SDHB is helpful in the identification of phaeochromocytomas, paragangliomas and GIST.
Marker
Pheochromocytoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07786).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for SDHB Antibody (SDHB/2126) - Azide and BSA Free
Western Blot: SDHB Antibody (SDHB/2126)Azide and BSA Free [NBP3-08809]
Western Blot: SDHB Antibody (SDHB/2126) - Azide and BSA Free [NBP3-08809] - Western Blot Analysis of 1) 293; 2) Jurkat and 3) HepG2 Cell Lysate with SDHB Mouse Monoclonal Antibody (SDHB/2126).![Immunohistochemistry-Paraffin: SDHB Antibody (SDHB/2126) - Azide and BSA Free [NBP3-08809]](https://resources.bio-techne.com/images/products/SDHB-Antibody-SDHB-2126-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08809-img0001.jpg "Immunohistochemistry-Paraffin: SDHB Antibody (SDHB/2126) - Azide and BSA Free [NBP3-08809]")
Immunohistochemistry-Paraffin: SDHB Antibody (SDHB/2126) - Azide and BSA Free [NBP3-08809]
Immunohistochemistry-Paraffin: SDHB Antibody (SDHB/2126) - Azide and BSA Free [NBP3-08809] - Formalin-fixed, paraffin-embedded human Kidney stained with SDHB Mouse Monoclonal Antibody (SDHB/2126).Applications for SDHB Antibody (SDHB/2126) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
0.5-1.0 ug/ml
Application Notes
Immunohistology Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: SDHB
Alternate Names
EC 1.3.5.1, FLJ92337, Ip, Iron-sulfur subunit of complex II, PGL4, SDH, SDH1, SDH2, SDHIP, succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial, succinate dehydrogenase complex, subunit B, iron sulfur (Ip)
Gene Symbol
SDHB
Additional SDHB Products
Product Documents for SDHB Antibody (SDHB/2126) - Azide and BSA Free
Product Specific Notices for SDHB Antibody (SDHB/2126) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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