SDHB Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-54154

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Bovine (92%), Porcine (92%), Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.14 mg/ml

View all SDHB Antibodies »

Product Specifications

Immunogen

A genomic peptide made to an N-terminal region of the human SDHB protein (within residues 1-150). [Swiss-Prot P21912]

Reactivity Notes

Immunogen sequence has 92% identity to rat, cow and pig, 85% identity to Xenopus and 84% identity to Zebrafish.

Localization

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.

Marker

Mitochondria Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SDHB Antibody - BSA Free

Western Blot: SDHB AntibodyBSA Free [NBP1-54154]

Western Blot: SDHB AntibodyBSA Free [NBP1-54154]

Western Blot: SDHB Antibody [NBP1-54154] - analysis of SDHB in porcine whole skeletal muscle lysate using anti-SDHB antibody. The primary antibody was used at a dilution of 1:2000 and incubated for 18 hours at 4C. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: SDHB Antibody - BSA Free [NBP1-54154]

Immunocytochemistry/ Immunofluorescence: SDHB Antibody - BSA Free [NBP1-54154]

Immunocytochemistry/Immunofluorescence: SDHB Antibody [NBP1-54154] - ICC staining of SDHB in HeLa cells with FITC (green). Nuclei were counterstained with Dapi (blue).
Immunohistochemistry: SDHB Antibody - BSA Free [NBP1-54154]

Immunohistochemistry: SDHB Antibody - BSA Free [NBP1-54154]

Immunohistochemistry: SDHB Antibody [NBP1-54154] - IHC staining of SDHB in mouse bladder.
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Applications for SDHB Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:7000
Application Notes
This SDHB antibody is useful for ICC/IF, IHC and Western blot where a band is seen ~31 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reviewed Applications

Read 1 review rated 3 using NBP1-54154 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.14 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SDHB

SDHB [Succinate dehydrogenase (ubiquinone) iron-sulfur subunit, mitochondrial] is an iron-sulfur protein subunit of succinate dehydrogenase (SDH) which is involved in mitochondrial electron transport chain's complex II and is accountable for electrons transfer from succinate to ubiquinone/coenzyme Q. The complex II is a key respiratory enzyme which links Krebs cycle and electron transport chain, and is composed of four subunits: the flavoprotein SDHA, iron-sulfur protein SDHB, and a cytochrome b560 composed of SDHC and SDHD as well as additional groups such as iron-sulphur centres and ubiquinone. SDH complex functions as a tumour suppressor, and loss of any of the subunit proteins leads to destabilization of the complex that follows carcinogenesis. Defects in SDHB have also been linked to susceptibility to pheochromocytoma (PCC), paragangliomas type 4 (PGL4), paraganglioma and gastric stromal sarcoma (PGGSS), and Cowden-like syndrome (CWDLS).

Alternate Names

EC 1.3.5.1, FLJ92337, Ip, Iron-sulfur subunit of complex II, PGL4, SDH, SDH1, SDH2, SDHIP, succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial, succinate dehydrogenase complex, subunit B, iron sulfur (Ip)

Entrez Gene IDs

6390 (Human); 67680 (Mouse)

Gene Symbol

SDHB

UniProt

P21912 (Human), Q9CQA3 (Mouse)

Additional SDHB Products

Product Documents for SDHB Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SDHB Antibody - BSA Free

Manufactured by Genomic Antibody Technology™. GAT FAQs

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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