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SEC13 Antibody (1G7)

Novus Biologicals, part of Bio-Techne | Catalog # H00006396-M02

Novus Biologicals, part of Bio-Techne
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H00006396-M02

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1G7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

SEC13 (NP_109598, 226 a.a. ~ 324 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SIGLPTSTIASCSQDGRVFIWTCDDASSNTWSPKLLHKFNDVVWHVSWSITANILAVSGGDNKVTLWKESVDGQWVCISDVNKGQGSVSASVTEGQQNE

Specificity

Reacts with SEC13 homolog (S. cerevisiae).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for SEC13 Antibody (1G7)

Immunocytochemistry/ Immunofluorescence: SEC13 Antibody (1G7) [H00006396-M02]

Immunocytochemistry/ Immunofluorescence: SEC13 Antibody (1G7) [H00006396-M02]

Immunocytochemistry/Immunofluorescence: SEC13L1 Antibody (1G7) [H00006396-M02] - Analysis of monoclonal antibody to SEC13 on HeLa cell . Antibody concentration 40 ug/ml.
Sandwich ELISA: SEC13L1 Antibody (1G7) [H00006396-M02] - Detection limit for recombinant GST tagged SEC13 is 0.03 ng/ml as a capture antibody.
Immunoprecipitation: SEC13 Antibody (1G7) [H00006396-M02]

Immunoprecipitation: SEC13 Antibody (1G7) [H00006396-M02]

Immunoprecipitation: SEC13L1 Antibody (1G7) [H00006396-M02] - Analysis of SEC13 transfected lysate using anti-SEC13 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SEC13 MaxPab rabbit polyclonal antibody.

Applications for SEC13 Antibody (1G7)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SEC13

The protein encoded by this gene belongs to the SEC13 family of WD-repeat proteins. It is a constituent of the endoplasmic reticulum and the nuclear pore complex. It has similarity to the yeast SEC13 protein, which is required for vesicle biogenesis from endoplasmic reticulum during the transport of proteins. Multiple alternatively spliced transcript variants have been found. [provided by RefSeq]

Long Name

Nuclear Pore Complex Protein Nup85

Alternate Names

D3S1231E, Npp-20, SEC13L1, SEC13R

Entrez Gene IDs

6396 (Human)

Gene Symbol

SEC13

Additional SEC13 Products

Product Documents for SEC13 Antibody (1G7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SEC13 Antibody (1G7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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