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SEC13 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33291

Novus Biologicals, part of Bio-Techne
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NBP1-33291

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (96%), Chicken (94%), Mouse (97%), Primate (99%), Rat (97%), Rhesus Macaque (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human SEC13. The exact sequence is proprietary.

Reactivity Notes

Chimpanzee (99%), Zebrafish (87%), Xenopus laevis (88%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SEC13 Antibody

Immunocytochemistry/ Immunofluorescence: SEC13 Antibody [NBP1-33291]

Immunocytochemistry/ Immunofluorescence: SEC13 Antibody [NBP1-33291]

Immunocytochemistry/Immunofluorescence: SEC13 Antibody [NBP1-33291] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SEC13L1 protein stained by SEC13L1 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: SEC13 Antibody [NBP1-33291]

Immunohistochemistry-Paraffin: SEC13 Antibody [NBP1-33291]

Immunohistochemistry-Paraffin: SEC13 Antibody [NBP1-33291] - Paraffin-embedded A549 xenograft, using antibody at 1:100 dilution.
Immunoprecipitation: SEC13 Antibody [NBP1-33291]

Immunoprecipitation: SEC13 Antibody [NBP1-33291]

Immunoprecipitation: SEC13 Antibody [NBP1-33291] - HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of SEC13L1 protein expressing HepG2 cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of SEC13L1 by 2.5 ug of SEC13L1 antibody 10% SDS-PAGE The immunoprecipitated SEC13L1 protein was detected by SEC13L1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.

Applications for SEC13 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SEC13

The protein encoded by this gene belongs to the SEC13 family of WD-repeat proteins. It has similarity to the yeast SEC13 protein, which is required for vesicle biogenesis from endoplasmic reticulum during the transport of proteins.

Long Name

Nuclear Pore Complex Protein Nup85

Alternate Names

D3S1231E, Npp-20, SEC13L1, SEC13R

Gene Symbol

SEC13

Additional SEC13 Products

Product Documents for SEC13 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SEC13 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.