SEC13 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33291
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (96%), Chicken (94%), Mouse (97%), Primate (99%), Rat (97%), Rhesus Macaque (98%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human SEC13. The exact sequence is proprietary.
Reactivity Notes
Chimpanzee (99%), Zebrafish (87%), Xenopus laevis (88%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SEC13 Antibody
Immunocytochemistry/ Immunofluorescence: SEC13 Antibody [NBP1-33291]
Immunocytochemistry/Immunofluorescence: SEC13 Antibody [NBP1-33291] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SEC13L1 protein stained by SEC13L1 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.Immunohistochemistry-Paraffin: SEC13 Antibody [NBP1-33291]
Immunohistochemistry-Paraffin: SEC13 Antibody [NBP1-33291] - Paraffin-embedded A549 xenograft, using antibody at 1:100 dilution.Immunoprecipitation: SEC13 Antibody [NBP1-33291]
Immunoprecipitation: SEC13 Antibody [NBP1-33291] - HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of SEC13L1 protein expressing HepG2 cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of SEC13L1 by 2.5 ug of SEC13L1 antibody 10% SDS-PAGE The immunoprecipitated SEC13L1 protein was detected by SEC13L1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.Applications for SEC13 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: SEC13
Long Name
Nuclear Pore Complex Protein Nup85
Alternate Names
D3S1231E, Npp-20, SEC13L1, SEC13R
Gene Symbol
SEC13
Additional SEC13 Products
Product Documents for SEC13 Antibody
Product Specific Notices for SEC13 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.