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Secretagogin Antibody (CL0271)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52897

Novus Biologicals, part of Bio-Techne
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NBP2-52897
NBP2-52897-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0271

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: RDLFLHHKKAISEAKLEEYTGTMMKIFDRNKDGRLDLNDLARILALQENFLLQFKMDACSTEERKRDFEKIFAYYDVSKTGALEGPEVDGFVKDMMELVQPSISGVDLDKFREILLRHCDVNKDGKIQKSELALCLGLK

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Secretagogin Antibody (CL0271)

Western Blot: Secretagogin Antibody (CL0271) [NBP2-52897]

Western Blot: Secretagogin Antibody (CL0271) [NBP2-52897]

Western Blot: Secretagogin Antibody (CL0271) [NBP2-52897] - Lane 1: Marker [kDa], Lane 2: Human brain tissue lysate
Immunohistochemistry-Paraffin: Secretagogin Antibody (CL0271) [NBP2-52897]

Immunohistochemistry-Paraffin: Secretagogin Antibody (CL0271) [NBP2-52897]

Immunohistochemistry-Paraffin: Secretagogin Antibody (CL0271) [NBP2-52897] - Staining of human pancreas shows strong cytoplasmic and nuclear immunoreactivity in the islets of Langerhans.
Immunohistochemistry-Paraffin: Secretagogin Antibody (CL0271) [NBP2-52897]

Immunohistochemistry-Paraffin: Secretagogin Antibody (CL0271) [NBP2-52897]

Immunohistochemistry-Paraffin: Secretagogin Antibody (CL0271) [NBP2-52897] - Staining of human cerebellum shows moderate nuclear positivity in the molecular layer.

Applications for Secretagogin Antibody (CL0271)

Application
Recommended Usage

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Secretagogin

The encoded protein is a secreted calcium-binding protein which is found in the cytoplasm. It is related to calbindin D-28K and calretinin. This protein is thought to be involved in KCL-stimulated calcium flux and cell proliferation.

Alternate Names

CALBL, SCGN, SECRET, SEGN, Setagin

Gene Symbol

SCGN

Additional Secretagogin Products

Product Documents for Secretagogin Antibody (CL0271)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Secretagogin Antibody (CL0271)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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