Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow (Cell Surface), Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # A8

Concentration

0.5 mg/ml

View all Semaphorin 4D/CD100 Antibodies »

Product Summary for Semaphorin 4D/CD100 Antibody (A8)

Immunogen

PHA-activated human lymphocytes were used as the immunogen for the CD100 antibody.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Semaphorin 4D/CD100 Antibody (A8)

Flow Cytometry: Semaphorin 4D/CD100 Antibody (A8) [NBP2-25198]

Flow Cytometry: Semaphorin 4D/CD100 Antibody (A8) [NBP2-25198]

Flow Cytometry: Semaphorin 4D/CD100 Antibody (A8) [NBP2-25198] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-25198. Surface Staining of CD100 in 10^6 human lymphocytes using 10 ul (0.25 ug) of CD100 antibody. Shaded histogram represents cells without antibody; green represents isotype control ; red represents CD100 antibody. surface staining flow kit was used for this test (cells were not fixed for testing).
Flow Cytometry: Semaphorin 4D/CD100 Antibody (A8) [NBP2-25198]

Flow Cytometry: Semaphorin 4D/CD100 Antibody (A8) [NBP2-25198]

Flow Cytometry: Semaphorin 4D/CD100 Antibody (A8) [NBP2-25198] - Cell surface analysis of CD100 in 10^6 human lymphocytes using 0.25 ug of NBP2-25198. Shaded histogram represents cells without antibody; green represents isotype control ; red represents anti-CD100 antibody. Goat anti-mouse IgG PE conjugated secondary was used in this test.
Flow (Cell Surface): Semaphorin 4D/CD100 Antibody (A8) [NBP2-25198]

Flow (Cell Surface): Semaphorin 4D/CD100 Antibody (A8) [NBP2-25198]

Flow (Cell Surface): Semaphorin 4D/CD100 Antibody (A8) [NBP2-25198] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-25198. Staining of CD100 in 10^6 human lymphocytes using 10 ul (0.25 ug) of this antibody. Shaded histogram represents cells without antibody; green represents isotype control ; red represents antibody.

Applications for Semaphorin 4D/CD100 Antibody (A8)

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunoprecipitation

1:10-1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

CD100 antibody at 0.5 mg/ml in PBS containing 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Semaphorin 4D/CD100

CD100 commonly known as SEMA4D is a cell surface protein of resting T cells involved in lymphocyte activation. An 862 amino acid signaling growth factor CD100 belongs to neuro-semaphorin family of proteins with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. Activation of plexin-B1 by CD100 induces different biological responses through the differential association of its receptor with ErbB-2 and Met. CD100 regulates certain immune responses by mediating in vitro and in vivo allo-geneic responses by modulating T cell-APC interactions and induces B cells aggregation, differentiation and improves their viability in vitro. Expression of CD100 on the surface of activated NK cells increases their cytotoxicity and mediates the killing of target cells by interacting with CD72. It is an important functional protein and a novel leukocyte semaphorin, of both the immune system, and the nervous system and is widely expressed in most of the tissues both hematopoietic and nonhematopoietic.

Alternate Names

A8, BB18, CD100, COLL-4, M-sema-G, SEMA4D, SEMAJ

Gene Symbol

SEMA4D

UniProt

NP_006369

Additional Semaphorin 4D/CD100 Products

Product Documents for Semaphorin 4D/CD100 Antibody (A8)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Semaphorin 4D/CD100 Antibody (A8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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