Semaphorin 4D/CD100 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86705

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-86705
NBP1-86705-25ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Semaphorin 4D/CD100 Antibodies »

Product Summary for Semaphorin 4D/CD100 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LLIGKKKPKSDFCDREQSLKETLVEPGSFSQQNGEHPKPALDTGYETEQDTITSKVPTDREDSQRIDDLSARDKPFDVKCELKFADSDADGD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Semaphorin 4D/CD100 Antibody

Western Blot: Semaphorin 4D/CD100 Antibody [NBP1-86705]

Western Blot: Semaphorin 4D/CD100 Antibody [NBP1-86705]

Western Blot: Semaphorin 4D/CD100 Antibody [NBP1-86705] - Analysis in human cell line HDLM-2.
Immunohistochemistry-Paraffin: Semaphorin 4D/CD100 Antibody [NBP1-86705]

Immunohistochemistry-Paraffin: Semaphorin 4D/CD100 Antibody [NBP1-86705]

Immunohistochemistry-Paraffin: Semaphorin 4D/CD100 Antibody [NBP1-86705] - Staining of human cervix shows moderate granular cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: Semaphorin 4D/CD100 Antibody [NBP1-86705]

Immunohistochemistry-Paraffin: Semaphorin 4D/CD100 Antibody [NBP1-86705]

Immunohistochemistry-Paraffin: Semaphorin 4D/CD100 Antibody [NBP1-86705] - Staining of human lymph node shows moderate membranous and cytoplasmic positivity in germinal and non-germinal center cells.
View 2 more images

Applications for Semaphorin 4D/CD100 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Semaphorin 4D/CD100

Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100, a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.

Alternate Names

A8, BB18, CD100, COLL-4, M-sema-G, SEMA4D, SEMAJ

Gene Symbol

SEMA4D

Additional Semaphorin 4D/CD100 Products

Product Documents for Semaphorin 4D/CD100 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Semaphorin 4D/CD100 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.