sen15 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89888

Novus Biologicals, part of Bio-Techne
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NBP1-89888
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all sen15 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EERGDSEPTPGCSGLGPDGVRGFGDGGGAPSWAPEDAWMGTHPKYLEMMELDIGDATHVYVAFLVYLDLMESK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (84%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for sen15 Antibody

Western Blot: sen15 Antibody [NBP1-89888]

Western Blot: sen15 Antibody [NBP1-89888]

Western Blot: sen15 Antibody [NBP1-89888] - Analysis in control (vector only transfected HEK293T lysate) and TSEN15 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: sen15 Antibody [NBP1-89888]

Immunocytochemistry/ Immunofluorescence: sen15 Antibody [NBP1-89888]

Immunocytochemistry/Immunofluorescence: sen15 Antibody [NBP1-89888] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli fibrillar center.
Immunohistochemistry-Paraffin: sen15 Antibody [NBP1-89888]

Immunohistochemistry-Paraffin: sen15 Antibody [NBP1-89888]

Immunohistochemistry-Paraffin: sen15 Antibody [NBP1-89888] - Staining of human esophagus shows distinct nucleolar positivity in squamous epithelial cells.

Applications for sen15 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: sen15

tRNA splicing is a fundamental process required for cell growth and division. SEN15 is a subunit of the tRNA splicing endonuclease, which catalyzes the removal of introns, the first step in tRNA splicing (Paushkin et al., 2004 [PubMed 15109492]).[supplied by OMIM]

Alternate Names

C1orf19, chromosome 1 open reading frame 19, hsSen15, SEN15, SEN15 homolog, tRNA splicing endonuclease 15 homolog (S. cerevisiae), tRNA-intron endonuclease Sen15, tRNA-splicing endonuclease subunit Sen15

Gene Symbol

TSEN15

Additional sen15 Products

Product Documents for sen15 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for sen15 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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