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Septin-3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90339

Novus Biologicals, part of Bio-Techne
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NBP1-90339
NBP1-90339-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LSKVVNIIPVIAKADTMTLEEKSEFKQRVRKELEVNGIEFYPQKEFDEDLEDKTENDKIRQESMPFAVVGSDKEYQVNGKRVLGRKTPWGIIEVENLNHCEFALLRDFVIRTHLQDLKEVTHNIHYETYRAKRLNDNGG

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Septin-3 Antibody

Western Blot: Septin-3 Antibody [NBP1-90339]

Western Blot: Septin-3 Antibody [NBP1-90339]

Western Blot: Septin-3 Antibody [NBP1-90339] - Analysis in control (vector only transfected HEK293T lysate) and LY403438 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: Septin-3 Antibody [NBP1-90339]

Immunohistochemistry-Paraffin: Septin-3 Antibody [NBP1-90339]

Immunohistochemistry-Paraffin: Septin-3 Antibody [NBP1-90339] - Staining in human cerebral cortex and liver tissues using anti-SEPT3 antibody. Corresponding SEPT3 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Septin-3 Antibody [NBP1-90339]

Immunohistochemistry-Paraffin: Septin-3 Antibody [NBP1-90339]

Immunohistochemistry-Paraffin: Septin-3 Antibody [NBP1-90339] - Staining of human cerebral cortex shows high expression.

Applications for Septin-3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Septin-3

Septin 3 belongs to the septin family of GTPases. Members of this family are required for cytokinesis. Expression is upregulated by retinoic acid in a human teratocarcinoma cell line. The specific function of this gene has not been determined. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Alternate Names

bK250D10.3, MGC133218, neuronal-specific septin-3, SEP3neuronal-specific septin 3, septin 3

Gene Symbol

SEPTIN3

Additional Septin-3 Products

Product Documents for Septin-3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Septin-3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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