Septin-4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52096
Key Product Details
Species Reactivity
Human, Mouse
Applications
Flow Cytometry, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-ELSKFVKDFSGN corresponding to internal region according to NP_004565.1, NP_536340.1, NP_536341.1, NP_001185642.1.
Epitope
C-ELSKFVKDFSGN
Specificity
This antibody is expected to recognize all reported isoforms (NP_004565.1; NP_536340.1; NP_536341.1, NP_001185642.1). Amino acid numbering in name refers to NP_004565.1 sequence.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Septin-4 Antibody
![Western Blot: Septin-4 Antibody [NBP1-52096]](https://resources.bio-techne.com/images/products/Septin-4-Antibody-Western-Blot-NBP1-52096-img0002.jpg "Western Blot: Septin-4 Antibody [NBP1-52096]")
Western Blot: Septin-4 Antibody [NBP1-52096]
Western Blot: Septin-4 Antibody [NBP1-52096] - (0.3ug/ml) staining of 1Human Cerebellum (A) and Mouse Brain (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.![Flow Cytometry: Septin-4 Antibody [NBP1-52096]](https://resources.bio-techne.com/images/products/Septin-4-Antibody-Flow-Cytometry-NBP1-52096-img0003.jpg "Flow Cytometry: Septin-4 Antibody [NBP1-52096]")
Flow Cytometry: Septin-4 Antibody [NBP1-52096]
Flow Cytometry: Septin-4 Antibody [NBP1-52096] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.![Western Blot: Septin-4 Antibody [NBP1-52096]](https://resources.bio-techne.com/images/products/Septin-4-Antibody-Western-Blot-NBP1-52096-img0001.jpg "Western Blot: Septin-4 Antibody [NBP1-52096]")
Western Blot: Septin-4 Antibody [NBP1-52096]
Western Blot: Septin-4 Antibody [NBP1-52096] - (0.3ug/ml) staining of Jurkat nuclear (A) and LNCaP (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.Applications for Septin-4 Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/ml
Peptide ELISA
Detection limit 1:16000
Western Blot
0.1-0.3 ug/mL
Application Notes
WB: Approx 55kDa band observed in nuclear lysates of cell lines Jurkat and in LNCaP cell lysates, 50-55kDa in Human Cerebellum lysates, and approx. 50kDa in Mouse Brain lysates (calculated MW of 53.0kDa according to Human NP_536341.1 and 49.4kDa according to Mouse NP_001271323.1).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Septin-4
Alternate Names
Apoptosis-related protein in the TGF-beta signaling pathway, ARTSPeanut-like protein 2, BRADEION, Bradeion beta, Brain protein H5, CE5B3, Cell division control-related protein 2, Cerebral protein 7, H5, hCDCREL-2SEP4, hucep-7, MART, peanut-like 2, peanut-like 2 (Drosophila), PNUTL2CE5B3 beta, septin 4, septin-4, septin-M
Gene Symbol
SEPTIN4
UniProt
Additional Septin-4 Products
Product Documents for Septin-4 Antibody
Product Specific Notices for Septin-4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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