SERCA1 ATPase Antibody (1B11)

Novus Biologicals, part of Bio-Techne | Catalog # H00000487-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1B11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SERCA1 ATPase Antibodies »

Product Specifications

Immunogen

ATP2A1 (NP_775293, 522 a.a. ~ 612 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. IDRCNYVRVGTTRVPLTGPVKEKIMAVIKEWGTGRDTLRCLALATRDTPPKREEMVLDDSARFLEYETDLTFVGVVGMLDPPRKEVTGSIQ

Specificity

ATP2A1 - ATPase, Ca++ transporting, cardiac muscle, fast twitch 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for SERCA1 ATPase Antibody (1B11)

Western Blot: SERCA1 ATPase Antibody (1B11) [H00000487-M01]

Western Blot: SERCA1 ATPase Antibody (1B11) [H00000487-M01]

Western Blot: SERCA1 ATPase Antibody (1B11) [H00000487-M01] - Analysis of ATP2A1 expression in transfected 293T cell line by ATP2A1 monoclonal antibody (M01), clone 1B11.Lane 1: ATP2A1 transfected lysate(109.3 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: SERCA1 ATPase Antibody (1B11) [H00000487-M01]

Immunocytochemistry/ Immunofluorescence: SERCA1 ATPase Antibody (1B11) [H00000487-M01]

Immunocytochemistry/Immunofluorescence: SERCA1 ATPase Antibody (1B11) [H00000487-M01] - Analysis of monoclonal antibody to ATP2A1 on A-431 cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: SERCA1 ATPase Antibody (1B11) [H00000487-M01]

Immunohistochemistry-Paraffin: SERCA1 ATPase Antibody (1B11) [H00000487-M01]

Immunohistochemistry-Paraffin: SERCA1 ATPase Antibody (1B11) [H00000487-M01] - Analysis of monoclonal antibody to ATP2A1 on formalin-fixed paraffin-embedded human cerebral cortex. Antibody concentration 3 ug/ml.
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Applications for SERCA1 ATPase Antibody (1B11)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SERCA1 ATPase

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise. Alternative splicing results in two transcript variants encoding different isoforms.

Long Name

Sarcoplasmic/endoplasmic reticulum calcium ATPase 1

Alternate Names

ATP2A1, Calcium pump 1, EC 7.2.2.10, SERCA1, SR Ca(2+)-ATPase 1

Entrez Gene IDs

487 (Human)

Gene Symbol

ATP2A1

OMIM

108730 (Human)

UniProt

NP_775293 (Human)

Additional SERCA1 ATPase Products

Product Documents for SERCA1 ATPase Antibody (1B11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SERCA1 ATPase Antibody (1B11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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