SERCA2 ATPase Antibody (IID8) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB300-529

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Amphibian, Bovine, Canine, Guinea Pig, Hamster, Primate, Rabbit, Sheep, Virus

Cited:

Human

Applications

Validated:

Block/Neutralize, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # IID8

Format

BSA Free

Concentration

1 mg/ml

View all SERCA2 ATPase Antibodies »

Product Specifications

Immunogen

Purified canine cardiac sarcoplasmic reticulum.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

Detects Sarcoplasmic or Endoplasmic Reticulum Calcium 2 (SERCA2) ATPase. This does not discriminate between the two isoforms.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SERCA2 ATPase Antibody (IID8) - BSA Free

Western Blot: SERCA2 ATPase Antibody (IID8)BSA Free [NB300-529]

Western Blot: SERCA2 ATPase Antibody (IID8)BSA Free [NB300-529]

Western Blot: SERCA2 ATPase Antibody (IID8) [NB300-529] - Analysis of 25 ug of rat skeletal muscle (lane 1) and human skeletal muscle (lane 2).
Immunocytochemistry/ Immunofluorescence: SERCA2 ATPase Antibody (IID8) - BSA Free [NB300-529]

Immunocytochemistry/ Immunofluorescence: SERCA2 ATPase Antibody (IID8) - BSA Free [NB300-529]

Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (IID8) [NB300-529] - SERCA2 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA2 ATPase at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (IID8) - BSA Free [NB300-529]

Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (IID8) - BSA Free [NB300-529]

Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (IID8) [NB300-529] - Both normal and cancer biopsies of deparaffinized human Heart tissue.
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Applications for SERCA2 ATPase Antibody (IID8) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1 ug / 10^6 cells

Immunocytochemistry/ Immunofluorescence

1:100 - 1:200

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:500

Immunohistochemistry-Paraffin

1 ug/ml

Western Blot

1:500 - 1:5000
Application Notes
WB: Detects an approx. 110 kDa protein representing SERCA2 ATPase from canine skeletal muscle triad preparations. Not recommended for WB detection of rat SERCA2. IF: Staining of SECRA2 ATPase in rabbit skeletal muscle results in strong labeling of the entire type I (slow) myofiber consistent with sarcoplasmic reticulum localization. May be useful in Flow.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SERCA2 ATPase

ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium (SERCA) ATPases. The SERCA1 gene is exclusively expressed in type II (fast) skeletal muscle. The SERCA2 gene is subject to tissue dependent processing which is responsible for the generation of SERCA2a muscle-specific form expressed in type I (slow) skeletal, cardiac and smooth muscle and the SERCA2b isoform expressed in all cell types. The SERCA3 gene is not as well characterized and is found in non-muscle cells.

Long Name

Sarcoplasmic/endoplasmic reticulum calcium ATPase 2

Alternate Names

ATP2A2, ATP2B, Calcium pump 2, SERCA2, SR Ca(2+)-ATPase 2

Entrez Gene IDs

488 (Human); 11938 (Mouse); 29693 (Rat); 403878 (Canine)

Gene Symbol

ATP2A2

UniProt

O46674 (Canine)

Additional SERCA2 ATPase Products

Product Documents for SERCA2 ATPase Antibody (IID8) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SERCA2 ATPase Antibody (IID8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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