Serpin B5/Maspin Antibody (SERPINB5/4975)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13733

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SERPINB5/4975

Concentration

0.2 mg/ml

View all Serpin B5/Maspin Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human Serpin B5/Maspin protein (around aa1-200) (exact sequence is proprietary) (Uniprot: P36952)

Localization

Secreted, Extracellular space

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14136)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Serpin B5/Maspin Antibody (SERPINB5/4975)

Western Blot: Serpin B5/Maspin Antibody (SERPINB5/4975) [NBP3-13733]

Western Blot: Serpin B5/Maspin Antibody (SERPINB5/4975) [NBP3-13733]

Western Blot: Serpin B5/Maspin Antibody (SERPINB5/4975) [NBP3-13733] - Western blot analysis of PC3 cell lysate using Serpin B5/Maspin antibody (SERPINB5/4975).
Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody (SERPINB5/4975) [NBP3-13733]

Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody (SERPINB5/4975) [NBP3-13733]

Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody (SERPINB5/4975) [NBP3-13733] - FFPE analysis of human cervical squamous cell carcinoma stained with Serpin B5/Maspin antibody (SERPINB5/4975). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody (SERPINB5/4975) [NBP3-13733]

Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody (SERPINB5/4975) [NBP3-13733]

Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody (SERPINB5/4975) [NBP3-13733] - Formalin-fixed, paraffin-embedded human skin stained with Serpin B5/Maspin antibody (SERPINB5/4975). Inset: PBS instead of primary antibody; secondary only negative control.
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Applications for Serpin B5/Maspin Antibody (SERPINB5/4975)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Serpin B5/Maspin

Maspin is an extracellular matrix tumor supressor that blocks the growth, invasion, and metastatic properties of mammary and prostate tumors. Structurally Maspin is a serine protease inhibitor (Serpin), but it does not undergo the stressed to relaxed conformational transition characteristic of active serpins and exhibits no serine protease inhibitory activity. Maspin is also expressed by the corneal epithelium and regulates migration and adhesion of corneal stromal fibroblasts and myofibroblasts during corneal wound healing.

Alternate Names

Maspin, PI5

Gene Symbol

SERPINB5

UniProt

P36952

Additional Serpin B5/Maspin Products

Product Documents for Serpin B5/Maspin Antibody (SERPINB5/4975)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Serpin B5/Maspin Antibody (SERPINB5/4975)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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