Serpin F1/PEDF Antibody (JE41-62)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32733
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE41-62
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human Serpin F1/PEDF aa 1-50 / 418. (Uniprot: P36955)
Localization
Secreted, Melanosome.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Serpin F1/PEDF Antibody (JE41-62)
Western Blot: Serpin F1/PEDF Antibody (JE41-62) [NBP3-32733]
Western Blot: Serpin F1/PEDF Antibody (JE41-62) [NBP3-32733] - Western blot analysis of Serpin F1/PEDF on different lysates with Rabbit anti-Serpin F1/PEDF antibody (NBP3-32733) at 1/1,000 dilution.Lane 1: Mouse liver tissue lysate
Lane 2: Rat liver tissue lysate
Lysates/proteins at 40 ug/Lane.
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32733) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.
Applications for Serpin F1/PEDF Antibody (JE41-62)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Serpin F1/PEDF
Long Name
Serpine F1/Pigment Epithelium-derived Factor
Alternate Names
EPC-1, PEDF
Gene Symbol
SERPINF1
Additional Serpin F1/PEDF Products
Product Documents for Serpin F1/PEDF Antibody (JE41-62)
Product Specific Notices for Serpin F1/PEDF Antibody (JE41-62)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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