Serum Amyloid A1 Antibody (20G5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26337

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 20G5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Serum Amyloid A1 Antibodies »

Product Summary for Serum Amyloid A1 Antibody (20G5)

Immunogen

A synthesized peptide derived from Human Serum Amyloid A1 [UniProt P0DJI8]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Serum Amyloid A1 Antibody (20G5)

Serum Amyloid A1 Antibody (20G5)

Flow Cytometry: Serum Amyloid A1 Antibody (20G5) [NBP3-26337] -

Flow Cytometry: Serum Amyloid A1 Antibody (20G5) [NBP3-26337] - Overlay Peak curve showing HepG2 cells stained with Serum Amyloid A1 Antibody (20G5) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for Serum Amyloid A1 Antibody (20G5)

Application
Recommended Usage

Flow Cytometry

1:50-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Serum Amyloid A1

The serum amyloid A (SAA) family comprises a number of differentially expressed lipoproteins, acute phase SAA1 and SAA2, the former being a major component in plasma, and constitutive SAA's (C-SAAs). Although the liver is the primary site of synthesis of both SAA types, extrhepatic production has been reported. The in vivo concentrations increase by as much as 1000 fold during inflammation. Several studies have expressed its importance in the diagnosis and monitoring of various diseases. Pathological SAA values are often detected in association with normal CRP concentrations. SAA rises earlier and more sharply than CRP. SAA enhances the binding of HDL's to macrophages and thus helps the delivery of lipid to sites of injury for use in tissue repair. It is thus thought to be an integral part of the disease process. In addition, recent experiments suggest that SAA may play a quot houekeeping quot role in normal human tissues. Elevated levels of SAA over time predispose secondary amyloidosis, extracellular accumulation of amyloid fibrils, derived from a circulating precursor, in various tissues and organs. The most common form of amyloidosis occurs secondary to chronic inflammatory disease, particularly rheumatoid artheritis. Amyloidosis is a heterogeneous group of disorders characterized by extracellular deposition of abnormal protein fibrils, which are derived from different proteins. Almost all patients with amyloidosis have some level of renal disorders. The immunoperoxidase technique has the advantage of being able to detect amyloid in formalin-fixed paraffin embedded tissues and is a very specific method for identifying amyloid fibril proteins. Small and minute amounts of amyloid can be detected with both Amyloid P (ab688) and Amyloid A antibodies, and thus could allow earlier treatment before organ damage has occurred.

Alternate Names

PIG4, SAA1

Gene Symbol

SAA1

Additional Serum Amyloid A1 Products

Product Documents for Serum Amyloid A1 Antibody (20G5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Serum Amyloid A1 Antibody (20G5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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