This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Serum Amyloid A4 Antibody (001)

Western Blot: Serum Amyloid A4 Antibody (001) [NBP2-89887]

Western Blot: Serum Amyloid A4 Antibody (001) [NBP2-89887] - Lane A: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:15 kDa Observed band size:14 kDa

Immunocytochemistry/ Immunofluorescence: Serum Amyloid A4 Antibody (001) [NBP2-89887]

Immunocytochemistry/Immunofluorescence: Serum Amyloid A4 Antibody (001) [NBP2-89887] - Staining of Human SAA4 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SAA4 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Flow Cytometry: Serum Amyloid A4 Antibody (001) [NBP2-89887]

Flow Cytometry: Serum Amyloid A4 Antibody (001) [NBP2-89887] - Analysis of Human SAA4 expression on HepG2 cells. The cells were treated, stained with purified anti-Human SAA4, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Applications for Serum Amyloid A4 Antibody (001)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Serum Amyloid A4

Major acute phase reactant. Apolipoprotein of the HDL complex

Alternate Names

C-SAA, SAA4, SAA5

Gene Symbol

SAA4

Additional Serum Amyloid A4 Products

Product Documents for Serum Amyloid A4 Antibody (001)

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Serum Amyloid A4 Antibody (001)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Serum Amyloid A4 Antibody (001)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89887

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

Concentration

View all Serum Amyloid A4 Antibodies »

Product Specifications

Immunogen

Clonality

Host

Isotype

Description

Scientific Data Images for Serum Amyloid A4 Antibody (001)

Western Blot: Serum Amyloid A4 Antibody (001) [NBP2-89887]

Immunocytochemistry/ Immunofluorescence: Serum Amyloid A4 Antibody (001) [NBP2-89887]

Flow Cytometry: Serum Amyloid A4 Antibody (001) [NBP2-89887]

Applications for Serum Amyloid A4 Antibody (001)

Immunocytochemistry/ Immunofluorescence

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: Serum Amyloid A4

Alternate Names

Gene Symbol

Additional Serum Amyloid A4 Products

Product Documents for Serum Amyloid A4 Antibody (001)

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Serum Amyloid A4 Antibody (001)