SETD7/9 Antibody (s4E5) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-04298

Novus Biologicals, part of Bio-Techne
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NBP1-04298
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # s4E5

Format

BSA Free

Concentration

1.0 mg/ml

View all SETD7/9 Antibodies »

Product Summary for SETD7/9 Antibody (s4E5) - BSA Free

Immunogen

Recombinant human SETD7/9 (1-366aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for SETD7/9 Antibody (s4E5) - BSA Free

Western Blot: SETD7/9 Antibody (s4E5)BSA Free [NBP1-04298]

Western Blot: SETD7/9 Antibody (s4E5)BSA Free [NBP1-04298]

Western Blot: SETD7/9 Antibody (s4E5) [NBP1-04298] - The Recombinant human SET7/9 protein was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SET7/9 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunocytochemistry/ Immunofluorescence: SETD7/9 Antibody (s4E5) - BSA Free [NBP1-04298]

Immunocytochemistry/ Immunofluorescence: SETD7/9 Antibody (s4E5) - BSA Free [NBP1-04298]

Immunocytochemistry/Immunofluorescence: SETD7/9 Antibody (s4E5) [NBP1-04298] - Analysis of SET7/9 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SET7/9 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Flow Cytometry: SETD7/9 Antibody (s4E5) - BSA Free [NBP1-04298]

Flow Cytometry: SETD7/9 Antibody (s4E5) - BSA Free [NBP1-04298]

Flow Cytometry: SETD7/9 Antibody (s4E5) [NBP1-04298] - Analysis of SET7/9 in Jurkat cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
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Applications for SETD7/9 Antibody (s4E5) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:250-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SETD7/9

Set7/9 is a histone methyltransferase(HMTase) that transfers methyl group to Lys4 of histone H3, in complex with S-adenosyl-L-methionine(AdoMet). The methylation of lysine residues of histones plays a critical role in the regulation of chromatin structure and gene expression.Acetylation, phosphorylation and methylation of the amino-terminal tails of histone are thought to be involved in the regulation of chromatin structure and function. The enzymes identified in the methylation of specific lysine residue on histones belong to the SET family with just one exception. Set7/9, unlike most other SET proteins, is exclusively a mono-methylase.

Alternate Names

EC 2.1.1.43, H3-K4-HMTase SETD7, histone H3-lysine 4-specific methyltransferase, histone-lysine N-methyltransferase SETD7, KIAA1717SET domain-containing protein 7, KMT7FLJ21193, Lysine N-methyltransferase 7, SET domain containing (lysine methyltransferase) 7, SET7/9Histone H3-K4 methyltransferase SETD7, SET7Set9, SET9

Gene Symbol

SETD7

UniProt

NP_085151 (Human)

Additional SETD7/9 Products

Product Documents for SETD7/9 Antibody (s4E5) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SETD7/9 Antibody (s4E5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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