SETD8 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91269

Novus Biologicals, part of Bio-Techne
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NBP1-91269
NBP1-91269SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

ICC/IF (Negative), Immunohistochemistry (Negative), Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.15 mg/ml

View all SETD8 Antibodies »

Product Summary for SETD8 Antibody - BSA Free

Immunogen

A synthetic peptide made to an internal portion of the human SETD8 protein (between residues 100-200) [UniProt Q9NQR1]

Localization

Nucleus. Chromosome. Note: Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SETD8 Antibody - BSA Free

Western Blot: SETD8 AntibodyBSA Free [NBP1-91269]

Western Blot: SETD8 AntibodyBSA Free [NBP1-91269]

Western Blot: SETD8 Antibody [NBP1-91269] - Analysis of SETD8 in whole cell lysates of mouse proliferating neural progenitors (P) and differentiating neural stem cell (D) using anti-SETD8 antibody. Image from verified customer review.
Western Blot: SETD8 AntibodyBSA Free [NBP1-91269]

Western Blot: SETD8 AntibodyBSA Free [NBP1-91269]

Western Blot: SETD8 Antibody [NBP1-91269] - Western blot analysis of SETD8 in Lane 1. SETD8 overexpression vector containing C-terminal DDK tag. Lane 2. Empty vector.

Applications for SETD8 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:1000
Application Notes
This SETD8 antibody is useful for Western Blot where a band is seen ~42 kDa representing SETD8 protein. This antibody is not applicable for Immunocytochemistry or Immunohistochemistry.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using NBP1-91269 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.15 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SETD8

SETD8 (KMT5A, SET8, PR-Set7, SET domain containing (lysine methyltransferase) 8) is a methyltransferase that appears to be involved in cell cycle regulation; primarily during G2/M phase. A recent study has shown that KMT5A is regulated by phosphorylation at Ser 29 by the cyclin-dependent kinase 1/cyclinB complex from prophase through early anaphase following accumulation of H4K20me1. While the phosphorylation did not alter KMT5A methyltransferase activity, it did cause the removal of KMT5A from mitotic chromosomes. This study also showed that dephosphorylation of Ser 29 in KMT5A during late mitosis is vital to proper mitotic progression. If Ser 29 is not dephosphorylated at the proper time, a delay between metaphase and anaphase occurs (PMID: 20966048). Another study showed that KMT5A binds to the H4 N-terminal tail, consequently blocking the acetylation of lysine residues K5, K8 and K12 of histone H4 during G1, which can inhibit DNA replication (PMID: 18418072).

Long Name

N-lysine methyltransferase KMT5A

Alternate Names

H4-K20-HMTase KMT5A, KMT5A, PR-Set7, PR/SET07, PRSET7, SET07, SET8

Entrez Gene IDs

387893 (Human)

Gene Symbol

KMT5A

UniProt

Q9NQR1 (Human)

Additional SETD8 Products

Product Documents for SETD8 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SETD8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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