SETD8 Antibody [DyLight 488]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91269G

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

ICC/IF (Negative), Immunohistochemistry (Negative), Western Blot

Label

DyLight 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SETD8 Antibodies »

Product Summary for SETD8 Antibody [DyLight 488]

Immunogen

A synthetic peptide made to an internal portion of the human SETD8 protein (between residues 100-200) [UniProt Q9NQR1]

Localization

Nucleus. Chromosome. Note: Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for SETD8 Antibody [DyLight 488]

Application
Recommended Usage

Western Blot

1:1000
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: SETD8

SETD8 (KMT5A, SET8, PR-Set7, SET domain containing (lysine methyltransferase) 8) is a methyltransferase that appears to be involved in cell cycle regulation; primarily during G2/M phase. A recent study has shown that KMT5A is regulated by phosphorylation at Ser 29 by the cyclin-dependent kinase 1/cyclinB complex from prophase through early anaphase following accumulation of H4K20me1. While the phosphorylation did not alter KMT5A methyltransferase activity, it did cause the removal of KMT5A from mitotic chromosomes. This study also showed that dephosphorylation of Ser 29 in KMT5A during late mitosis is vital to proper mitotic progression. If Ser 29 is not dephosphorylated at the proper time, a delay between metaphase and anaphase occurs (PMID: 20966048). Another study showed that KMT5A binds to the H4 N-terminal tail, consequently blocking the acetylation of lysine residues K5, K8 and K12 of histone H4 during G1, which can inhibit DNA replication (PMID: 18418072).

Long Name

N-lysine methyltransferase KMT5A

Alternate Names

H4-K20-HMTase KMT5A, KMT5A, PR-Set7, PR/SET07, PRSET7, SET07, SET8

Gene Symbol

KMT5A

Additional SETD8 Products

Product Documents for SETD8 Antibody [DyLight 488]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SETD8 Antibody [DyLight 488]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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