Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all SETDB1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 1125 and 1175 of human SET Domain, Bifurcated 1 using the numbering given in entry NP_036564.2 (GeneID 9869).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SETDB1 Antibody

Western Blot: SETDB1 Antibody [NB100-79776]

Western Blot: SETDB1 Antibody [NB100-79776]

Western Blot: SETDB1 Antibody [NB100-79776] - Detection of human SETDB1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SETDB1 antibody NB100-79776 used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: SETDB1 Antibody [NB100-79776]

Western Blot: SETDB1 Antibody [NB100-79776]

Western Blot: SETDB1 Antibody [NB100-79776] - HeLa whole cell lysate using NB100-79776. Rabbit anti-SETDB1 antibodies BL538 and NB100-79776 were used at 1 mcg/mg lysate for IP (B).
Western Blot: SETDB1 Antibody [NB100-79776]

Western Blot: SETDB1 Antibody [NB100-79776]

Western Blot: SETDB1 Antibody [NB100-79776] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SetDB1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for SETDB1 Antibody

Application
Recommended Usage

Immunoprecipitation

1-4 ug/mg of lysate

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SETDB1

The SET domain is a highly conserved, approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger conserved region present in the Drosophila Trithorax protein and was subsequently identified in the Drosophila Su(var)3-9 and 'Enhancer of zeste' proteins, from which the acronym SET is derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities. [supplied by OMIM]

Alternate Names

ERG-associated protein with SET domain, ESETEC 2.1.1.43, H3-K9-HMTase 4, Histone H3-K9 methyltransferase 4, histone-lysine N-methyltransferase SETDB1, histone-lysine N-methyltransferase, H3lysine-9 specific 4, KG1T, KIAA0067H3-K9-HMTase4, KMT1EERG-associated protein with a SET domain, ESET, Lysine N-methyltransferase 1E, SET domain bifurcated 1, SET domain, bifurcated 1

Entrez Gene IDs

9869 (Human)

Gene Symbol

SETDB1

UniProt

Q15047 (Human)

Additional SETDB1 Products

Product Documents for SETDB1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SETDB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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