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SF3A3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87212

Novus Biologicals, part of Bio-Techne
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NBP1-87212
NBP1-87212-25ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TKKSTLRDQINSDHRTRAMQDRYMEVSGNLRDLYDDKDGLRKEELNAISGPNEFAEFYNRLKQIKEFHRKHPNEICVPMSVEFE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SF3A3 Antibody

Western Blot: SF3A3 Antibody [NBP1-87212]

Western Blot: SF3A3 Antibody [NBP1-87212]

Western Blot: SF3A3 Antibody [NBP1-87212] - Analysis in human cell line U-251 MG and human cell line MCF-7.
Immunocytochemistry/ Immunofluorescence: SF3A3 Antibody [NBP1-87212]

Immunocytochemistry/ Immunofluorescence: SF3A3 Antibody [NBP1-87212]

Immunocytochemistry/Immunofluorescence: SF3A3 Antibody [NBP1-87212] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: SF3A3 Antibody [NBP1-87212]

Immunohistochemistry-Paraffin: SF3A3 Antibody [NBP1-87212]

Immunohistochemistry-Paraffin: SF3A3 Antibody [NBP1-87212] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.

Applications for SF3A3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SF3A3

The SF3A3 gene encodes subunit 3 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicin

Alternate Names

PRP9, PRPF9, SAP 61, SAP61splicing factor 3a, subunit 3, 60kD, SF3a60pre-mRNA splicing factor SF3a (60kD), spliceosome associated protein 61, Spliceosome-associated protein 61, splicing factor 3A subunit 3, splicing factor 3a, subunit 3, 60kDa

Gene Symbol

SF3A3

Additional SF3A3 Products

Product Documents for SF3A3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SF3A3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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