Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all sFRP-2 Antibodies »

Product Summary for sFRP-2 Antibody

Immunogen

Synthetic peptide from the C-terminal of Human Secreted frizzled-related protein 2 (aa. 195-295)

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for sFRP-2 Antibody

Western Blot: sFRP-2 Antibody [NBP3-18244]

Western Blot: sFRP-2 Antibody [NBP3-18244]

Western Blot: sFRP-2 Antibody [NBP3-18244] - Western blot analysis of Mouse Brain showing detection of 33.5 kDa sFRP-2 protein using Rabbit Anti-sFRP-2 Polyclonal Antibody (NBP3-18244). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain . Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-sFRP-2 Polyclonal Antibody (NBP3-18244) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 33.5 kDa. Other Band(s): 37 kDa.
Immunohistochemistry: sFRP-2 Antibody [NBP3-18244]

Immunohistochemistry: sFRP-2 Antibody [NBP3-18244]

Immunohistochemistry: sFRP-2 Antibody [NBP3-18244] - Immunohistochemistry analysis using Rabbit Anti-sFRP-2 Polyclonal Antibody (NBP3-18244). Tissue: Liver. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-sFRP-2 Polyclonal Antibody (NBP3-18244) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.

Applications for sFRP-2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50

Western Blot

1:1000
Application Notes
Optimal dilutions for assays should be determined by the user.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS pH 7.4, 50% glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: sFRP-2

SFRP2 encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. Methylation of this gene is a potential marker for the presence of colorectal cancer. [provided by RefSeq]

Long Name

Secreted Frizzled Related Protein 2

Alternate Names

SARP-1, SDF-5, sFRP2

Gene Symbol

SFRP2

Additional sFRP-2 Products

Product Documents for sFRP-2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for sFRP-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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