SGK1 Antibody (SC05-71)

Western Blot: SGK1 Antibody (SC05-71) [NBP2-67561]

Western Blot: SGK1 Antibody (SC05-71) [NBP2-67561] - Western blot analysis of SGK1 on human kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat

Immunohistochemistry-Paraffin: SGK1 Antibody (SC05-71) [NBP2-67561]

Immunohistochemistry-Paraffin: SGK1 Antibody (SC05-71) [NBP2-67561] - Analysis of paraffin-embedded mouse liver tissue using anti-SGK1 antibody. Counter stained with hematoxylin.

Flow Cytometry: SGK1 Antibody (SC05-71) [NBP2-67561]

Flow Cytometry: SGK1 Antibody (SC05-71) [NBP2-67561] - Flow cytometric analysis of SGK1 was done on 293 cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).