SH3BP2 Antibody (1E9)

Novus Biologicals, part of Bio-Techne | Catalog # H00006452-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1E9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SH3BP2 Antibodies »

Product Specifications

Immunogen

SH3BP2 (AAH22996, 452 a.a. ~ 561 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PLPNSVFVNTTESCEVERLFKATSPRGEPQDGLYCIRNSSTKSGKVLVVWDETSNKVRNYRIFEKDSKFYLEGEVLFVSVGSMVEHYHTHVLPSHQSLLLRHPYGYTGPR

Specificity

SH3BP2 - SH3-domain binding protein 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for SH3BP2 Antibody (1E9)

Western Blot: SH3BP2 Antibody (1E9) [H00006452-M01]

Western Blot: SH3BP2 Antibody (1E9) [H00006452-M01]

Western Blot: SH3BP2 Antibody (1E9) [H00006452-M01] - SH3BP2 monoclonal antibody (M01), clone 1E9 Analysis of SH3BP2 expression in A-431.
Immunocytochemistry/ Immunofluorescence: SH3BP2 Antibody (1E9) [H00006452-M01]

Immunocytochemistry/ Immunofluorescence: SH3BP2 Antibody (1E9) [H00006452-M01]

Immunocytochemistry/Immunofluorescence: SH3BP2 Antibody (1E9) [H00006452-M01] - Analysis of monoclonal antibody to SH3BP2 on A-431 cell. Antibody concentration 20 ug/ml.
Sandwich ELISA: SH3BP2 Antibody (1E9) [H00006452-M01] - Detection limit for recombinant GST tagged SH3BP2 is approximately 0.1ng/ml as a capture antibody.

Applications for SH3BP2 Antibody (1E9)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SH3BP2

The protein encoded by the SH3BP2 gene has an N-terminal pleckstrin homology (PH) domain, an SH3-binding proline-rich region, and a C-terminal SH2 domain. The protein binds to the SH3 domains of several proteins including the ABL1 and SYK protein tyrosine kinases , and functions as a cytoplasmic adaptor protein to positively regulate transcriptional activity in T, natural killer (NK), and basophilic cells. Mutations in this gene result in cherubism. Multiple transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq)

Alternate Names

Abl-SH3 binding protein 2, Cherubism, CRBM, CRPM, FLJ42079, FLJ54978,3BP-2,3BP2, RES4-23, SH3 domain-binding protein 2, SH3-domain binding protein 2, TNFAIP3 interacting protein 2

Entrez Gene IDs

6452 (Human)

Gene Symbol

SH3BP2

OMIM

118 (Human)

UniProt

AAH22996 (Human)

Additional SH3BP2 Products

Product Documents for SH3BP2 Antibody (1E9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SH3BP2 Antibody (1E9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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